ARUP's Laboratory Test Directory

Urticaria-Inducing Activity with Thyroid Antibodies and Stimulating Hormone : 2005415
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Additional Technical Information
  


Mnemonic: UIAT

Methodology: Semi-Quantitative Ex Vivo Challenge/Cell Culture/Quantitative Enzyme-Linked Immunosorbent Assay/Quantitative Chemiluminescent Immunoassay
Performed: Mon, Fri
Reported: 2-6 days
Specimen Required: Patient Prep: Patients taking calcineurin inhibitors should stop their medication 72 hours prior to draw. Patients on prednisone should be off their medication for 2 weeks prior to draw.

Collect: Plain red.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport tube and freeze immediately (Min: 0.5 mL) AND transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature: First Specimen: CRITICAL FROZEN. Separate specimens must be submitted for this multiple test panel.
Second Specimen: Refrigerated.

Unacceptable Conditions: Specimens other than serum. Contaminated, grossly hemolyzed, or lipemic specimens.

Stability (collection to initiation of testing): First Specimen: After separation from cells: Ambient: 30 minutes; Refrigerated: Unacceptable; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Second Specimen: After separation from cells: Ambient: 24 hours; Refrigerated: 1 Week; Frozen: 6 months
Reference Interval:
By report
Interpretive Data: Refer to report.

Refer to Statement B under Testing Information at http://www.aruplab.com.
Note: 1) Chronic urticaria (CU) is a common and complex dermatological condition that is suspected when patients experience persistent hives for over 6 weeks.  No published evidence of an exogenous allergen as the cause of this disorder exists. About 45 percent of cases have autoantibodies directed against either basophil or mast cell-associated IgE or the high affinity IgE-Fc receptor (Fc epsilon R1 alpha) (Clin Exp Allergy 2009; 39: 777-87). 

2) The presence of histamine releasing factors (including but not limited to IgE and Fc epsilon R1 alpha-specific autoantibodies) in the patient serum can be indirectly determined by evaluating basophil/mast cell activation status using histamine release assays, autologous serum-skin test, and flow cytometric measurement of the basophil and mast cell-specific marker CD203c. Serum from CU patients can activate donor basophils, which induces histamine release and CD203c upregulation (J Allergy Clin Immunol 2006; 117: 1430-4).
CPT Code(s): 86352; 86800; 84443; 86376
Cross References: Chronic Urticaria (Urticaria-Inducing Activity with Thyroid Antibodies and Stimulating Hormone), Chronic Urticaria Panel (Urticaria-Inducing Activity w/ Thyroid Antibodies and Stimulating Hormone)