ARUP's Laboratory Test Directory

EGFR Mutation Detection by PCR and Fragment Analysis : 2002440

Mnemonic: EGFR PCR

Methodology: Polymerase Chain Reaction/Fragment Analysis
Performed: Sun-Sat
Reported: 10-12 days
Specimen Required: Collect: Tumor tissue.

Specimen Preparation: Formalin fix (10% neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 5 unstained 5 micron slides. (Min: 3 slides) Protect paraffin block from excessive heat.

Storage/Transport Temperature: Room temperature or refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions: No tumor in tissue. Specimens fixed/processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5). Frozen specimens. Decalcified specimens.

Stability (collection to initiation of testing): Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Interpretive Data: Refer to report

This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

Refer to Statement B under Testing Information at http://www.aruplab.com.
Note: This test is designed to detect exon 19 deletions and the exon 21 L858R point mutation. These mutations account for about 90 percent of all reported EGFR mutations.
CPT Code(s): 88381 Microdissection; 83907 Lysis; 83898 x2 Amplification; 83892 Digestion; 83909 Separation and identification; 83896 x2 Nucleic acid probes; 83912 Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test oncologic or inherited disorders.
Cross References: ERBB1 (EGFR Mutation Detection by PCR and Fragment Analysis), Erlotinib Responsiveness in NSCLC (EGFR Mutation Detection by PCR and Fragment Analysis), Gefitinib Responsiveness in NSCLC (EGFR Mutation Detection by PCR and Fragment Analysis), IRESSA Responsiveness in NSCLC (EGFR Mutation Detection by PCR and Fragment Analysis), NSCLC or Non Small-Cell Lung Cancer (EGFR Mutation Detection by PCR and Fragment Analysis), Tarceva Responsiveness in NSCLC (EGFR Mutation Detection by PCR and Fragment Analysis)
 
 

 

 

 
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