ARUP's Laboratory Test Directory

EGFR Mutation Detection by Pyrosequencing : 2002440
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Additional Technical Information
  


Mnemonic: EGFR PCR

Methodology: Polymerase Chain Reaction/Pyrosequencing
Performed: Varies
Reported: 10-12 days
Specimen Required: Collect: Tumor tissue.

Specimen Preparation: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or 5 unstained 5-micron slides. (Min: 3 slides) Tissue block will be returned after testing.
Transport block and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature: Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions: Less than 25 percent tumor. Specimens fixed/processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives. Decalcified specimens

Stability (collection to initiation of testing): Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Interpretive Data: Refer to report.

See Compliance Statement B: www.aruplab.com/CS
Note: This test detects mutations in EGFR exons 18, 19, 20 and 21.
CPT Code(s): 88381, 81235, G0452
Cross References: ERBB1 (EGFR Mutation Detection by Pyrosequencing), Erlotinib Responsiveness in NSCLC (EGFR Mutation Detection by Pyrosequencing), Gefitinib Responsiveness in NSCLC (EGFR Mutation Detection by Pyrosequencing), IRESSA Responsiveness in NSCLC (EGFR Mutation Detection by Pyrosequencing), Non-small Cell Lung Cancer (NSCLC) Therapeutic Panel: EGFR Mutation Analysis and ALK FISH Analysis, Non-small Cell Lung Cancer (NSCLC) Therapeutic Panel: EGFR Mutation Analysis and ALK FISH Analysis (EGFR Mutation Detection by PCR and Fragment Analysis) , NSCLC or Non Small-Cell Lung Cancer (EGFR Mutation Detection by PCR and Fragment Analysis), Tarceva Responsiveness in NSCLC (EGFR Mutation Detection by PCR and Fragment Analysis)