ARUP's Laboratory Test Directory

25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum : 2002348

Mnemonic: VITD2D3TMS

Ordering Recommendation: Not the test of choice for initial evaluation of vitamin D insufficiency. Total vitamin D, 25-hydroxy (0080379) is preferred for diagnosis.
Methodology: Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
Performed: Sun-Sat
Reported: 1-4 days
Specimen Required: Collect: Plain red or serum separator tube. Also acceptable: Green (sodium heparin), lavender (EDTA), or pink (K2EDTA).

Specimen Preparation: Transfer 0.5 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Room temperature specimens older than 24 hours.

Stability (collection to initiation of testing): After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months
Reference Interval:
Effective May 16, 2011
1-17 years
Deficiency Less than 20 ng/mL
Optimum level Greater than or equal to 20 ng/mL*
*(Wagner CL et al. Pediatrics 2008; 122: 1142-52.)

18 years and older
Deficiency Less than 20 ng/mL
Insufficiency 20-29 ng/mL
Optimum Level 30-80 ng/mL
Possible Toxicity Greater than 150 ng/mL

Interpretive Data: Total Concentrations of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3:
Deficiency: Less than 20 ng/mL
Insufficiency: 20-29 ng/mL
Optimal Level: 30-80 ng/mL
Possible Toxicity: Greater than 150 ng/mL

See Compliance Statement B: www.aruplab.com/CS
Note: ARUP is unable to provide reliable results for specimens from infants (less than one year of age), since highly specialized test methodology is required.  ARUP will refer all infant specimens to a laboratory that is able to perform this methodology.
CPT Code(s): 82306
Cross References: 25-Hydroxy D2 (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , 25-Hydroxy D3 (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , 25-Hydroxy Vitamin D (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , 25-Hydroxycholecalciferol (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , 25-Hydroxyergocalciferol (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , 25-OH Vitamin D (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , Calcidiol (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , D2 (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , D3 (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , Fat Malabsorption (Response to Vitamin D2 Supplement) (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , Soft-25HDN (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , Total 25 Hydroxyvitamin D (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , Total 25-Hydroxycholecalciferol (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , Total Calcidiol (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , Total Calcifediol (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , Vitamin D (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , Vitamin D2 and D3 (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum) , Vitamin D3 (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)