ARUP's Laboratory Test Directory

Lymphocyte Antigen & Mitogen Proliferation Panel : 0096056
[ image for: Time Sensitive]
Time Sensitive
  


Mnemonic: LAM

Methodology: Cell Culture
Performed: Tue-Fri
Reported: 9-10 days
Specimen Required: Collect:  One 10 mL yellow (ACD Solution A) and a normal control in one 10 mL yellow (ACD Solution A) from a healthy unrelated individual.

Transport:  10 mL whole blood and 10 mL normal control in sterile containers at 20-25°C. LIVE LYMPHOCYTES REQUIRED. CRITICAL AMBIENT. Do not refrigerate or freeze.

Pediatric Collection/Transport:  3 mL whole blood (green, sodium heparin) and 3 mL whole blood (green, sodium heparin) normal control from a healthy unrelated individual in sterile containers at 20-25°C.

Remarks:  Patient and control samples must be collected within 48 hours of test performance.

Unacceptable Conditions:  Refrigerated or frozen samples or samples in transport longer than 48 hours.

Stability:  Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval:
By report
Interpretive Data: Phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen are tested independently in lymphocyte culture. Lymphocyte proliferation in response to these mitogens and antigens is determined by 3H-thymidine incorporation.

Results are reported as the counts per minute (CPM) mitogen or antigen stimulated versus a control culture and a stimulation index (SI) which represents the ratio of CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control. 

Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory.
Note: Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director.
CPT Code(s): 86353 x5 Lymphocyte transformation
 
 

 

 

 
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