ARUP's Laboratory Test Directory

Lymphocyte Transplantation CD3 : 0095949
[ image for: Time Sensitive]
Time Sensitive
  


Mnemonic: CD3

Methodology: Flow Cytometry
Performed: Sun-Sat
Reported: 1-2 days
Specimen Required: Collect:  One 5 mL green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).

Transport:  5 mL whole blood at 20-25°C. (Min: 0.5 mL)

Pediatric Collection/Transport:  0.5 mL whole blood at 20-25°C.

Remarks:  CRITICAL AMBIENT.  THIS TEST IS FOR TRANSPLANT PATIENTS ONLY. WBC and lymph % results with the same draw date and time as the CD3 sample must be provided by the client. Specimens must be analyzed within 48 hours of collection. Some medications may affect immunophenotyping results and should be listed on the patient test request form.
New York State Clients:
EDTA specimens must be analyzed within 30 hours of collection.  Heparin specimens must be analyzed within 48 hours of collection. 


Unacceptable Conditions:  Clotted, refrigerated, hemolyzed, or frozen specimens. Specimens older than 48 hours.
New York State Clients:
Clotted, refrigerated, hemolyzed, or frozen specimens.  EDTA specimens older than 30 hours and heparin specimens older than 48 hours.


Stability:  Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients:
  Ambient: EDTA specimens - 30 hours, Heparin specimens - 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval:
 
Components Age: 0-11 months Age: 12-23 months Age: 2 years and older
WBC (K/µL)
% Lymph
Absolute Lymph Count
Must be provided by the client.
5.4-15.7
50-78
3270-8840
4.9-14.8
44-72
2180-8270
3.1-8.4
22-57
1100-3400
%CD3
Absolute CD3
58-85
2170-6500
53-81
1460-5440
60-83
450-2300

Interpretive Data: In this test, the percent CD3 cells are those cells that are positive for both CD3 and the alpha-beta chain of the T-cell receptor and are presumed immunologically competent.

Please refer to Statement A in the Compliance Statements section in the front of the Laboratory Test Directory.
Note: THIS TEST IS FOR TRANSPLANT PATIENTS ONLY.  Effective immunosuppressive therapy with OKT3 can be documented by verifying CD3 antigen removal using this test.  For immunocompromised patients, order Lymphocyte Subset Panel 4 - T-Cell subsets Percent & Absolute (0095950).
CPT Code(s): 88184 First marker; 88185 Additional marker
 
 

 

 

 
All ARUP Sites:        www.aruplab.com     ·     www.arupconsult.com     ·     www.arup.utah.edu     ·     www.childx.org     ·     www.utahblood.org