ARUP's Laboratory Test Directory

HLA-B27 : 0095840

Mnemonic: HLAB27

Ordering Recommendation: Do not screen asymptomatic individuals. Aids in diagnosis of individuals with suspicious symptoms for ankylosing spondylitis or other related conditions.
Methodology: Qualitative Flow Cytometry
Performed: Sun-Sat
Reported: 1-2 days
Specimen Required: Collect: Lavender Hemogard (EDTA), pink Hemogard (K2EDTA), or green Hemogard (sodium or lithium heparin). Hemogard tubes are preferred for laboratory safety.

Specimen Preparation: Transport 4 mL whole blood. (Min: 0.5 mL)

Storage/Transport Temperature: CRITICAL ROOM TEMPERATURE.

Remarks: Specimens must be analyzed within 72 hours of collection.
New York State Clients: EDTA specimens must be analyzed within 30 hours of collection. Heparin specimens must be analyzed within 48 hours of collection.

Unacceptable Conditions: Frozen or refrigerated specimens. Specimens older than 72 hours. Clotted or hemolyzed specimens.
New York State Clients: Frozen or refrigerated specimens. Heparin specimens older than 48 hours or EDTA specimens older than 30 hours. Clotted or hemolyzed specimens.

Stability (collection to initiation of testing): Ambient: 72 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Ambient: 30 hours in EDTA, 48 hours in heparin; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval:
Negative
Interpretive Data: HLA-B27 is a serologically-defined allele of the human HLA-B locus. The presence of the HLA-B27 antigen is strongly associated with ankylosing spondylitis and related disorders.

The performance characteristics of this test were determined by ARUP Laboratories, Inc.

See Compliance Statement B: www.aruplab.com/CS
CPT Code(s): 86812
Cross References: Ankylosing Spondylitis (HLA-B27), HLA Antigen B27 (HLA-B27), Human Leukocyte Antigen, LY27 (HLA-B27)