ARUP's Laboratory Test Directory

0092356: Nicotine & Metabolites, Urine

Test Mnemonic: NICOTINEUR
Methodology: Tandem Mass Spectrometry

Performed: Sun-Sat

Reported: 1-4 days

Specimen Required:  
Collect: Random urine.

Transport: 10 mL urine at 20-25°C.

Pediatric Collection/Transport: 2.5 mL urine at 20-25°C.  

Unacceptable Conditions: Specimens exposed to repeated freeze/thaw cycles.

Stability: Ambient: 10 days; Refrigerated: 10 days; Frozen: 8 months

Reference Interval:
 
 Unexposed non-tobacco user Passive exposure Abstinent user for greater than 2 weeks Active tobacco product use
Nicotine Less than 2 ng/mL Less than 20 ng/mL Less than 30 ng/mL 1000-5000 ng/mL
Cotinine Less than 5 ng/mL Less than 20 ng/mL Less than 50 ng/mL 1000-8000 ng/mL
3-OH-Cotinine Less than 50 ng/mL Less than 50 ng/mL Less than 120 ng/mL 3000-25000 ng/mL
Nornicotine Less than 2 ng/mL Less than 2 ng/mL Less than 2 ng/mL 30-900 ng/mL
Anabasine Less than 2 ng/mL Less than 2 ng/mL Less than 2 ng/mL 3-500 ng/mL*
Adapted from Clinical Chemistry 2002;48:1460-71.

Interpretive Data:
Compounds covered: nicotine, cotinine, 3-OH-cotinine, nornicotine, and anabasine.
Positive cutoffs:
nicotine: 2 ng/mL
cotinine: 5 ng/mL
3-OH-cotinine: 50 ng/mL
nornicotine: 2 ng/mL
anabasine: 3 ng/mL
For medical purposes only; Not valid for forensic use.



CPT Code(s):
83887

 

 

 
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