ARUP's Laboratory Test Directory

Galactose-1-Phosphate in Red Blood Cells : 0081296
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Additional Technical Information
  


Mnemonic: GAL1P RBC

Methodology: Gas Chromatography-Mass Spectrometry
Performed: Tue
Reported: 2-9 days
Specimen Required: Collect: Lavender (EDTA) or green (sodium or lithium heparin).

Specimen Preparation: DO NOT FREEZE. Place tube on wet ice immediately after collection. Transport 5 mL whole blood. (Min: 2 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Frozen or room temperature specimens.

Stability (collection to initiation of testing): Ambient: Unacceptable; Refrigerated: 72 hours; Frozen: Unacceptable
Reference Interval:
Test Number Components Reference Interval
Galactose-1-phosphate 0.0-1.0 mg/dL
Galactose-1-phosphate (ug/g Hb) 0-53 ug/g Hb
Galactose-1-phosphate (umol/g Hb) 0.00-0.20 umol/g Hb

Interpretive Data:

See Compliance Statement B: www.aruplab.com/CS
Note: Alternate acceptable specimen is frozen, washed red blood cells.
1. Centrifuge whole blood immediately for 10 minutes at 800 g RCF.
2. Discard the plasma and buffy coat layers.
3. Add cold 0.9 percent saline solution to the red cells (about 2 times the volume of cells) and mix gently by inverting the tube.
4. Centrifuge for 10 minutes at 800 g RCF.
5. Aspirate and discard the supernatant.
6. Repeat the wash (steps 3 through 5) 2 more times.
7. After the 3rd wash and centrifugation, remove the supernatant and a thin layer of the top cells. 
8. Transfer washed cells to an ARUP standard transport tube and freeze. Ship on dry ice and include an ORDER COMMENT stating the specimen is washed, packed red blood cells.
 
Washed red cells must be frozen.  Ambient and refrigerated washed cells are unacceptable
CPT Code(s): 84378
Cross References: Gal1P (Galactose-1-Phosphate in Red Blood Cells), Galactose 1 (Galactose-1-Phosphate in Red Blood Cells), Galactose 1 Phosphate (Galactose-1-Phosphate in Red Blood Cells), Galactosemia Analyte (Galactose-1-Phosphate in Red Blood Cells), Galactosemia Metabolite(s) (Galactose-1-Phosphate in Red Blood Cells), Galactosemia Monitoring (Galactose-1-Phosphate in Red Blood Cells), Galactosemia Therapy (Galactose-1-Phosphate in Red Blood Cells), GALT Analyte (Galactose-1-Phosphate in Red Blood Cells), GALT Monitoring (Galactose-1-Phosphate in Red Blood Cells)