ARUP's Laboratory Test Directory

Herpes Simplex Virus Culture with Reflex to HSV Typing : 0065065

Mnemonic: V HSVCT

Ordering Recommendation: Aids in detection of herpes simplex virus (HSV) infection. Positive culture reflexes to HSV typing. Molecular testing is generally preferred.
Methodology: Cell Culture/Immunoassay
Performed: Sun-Sat
Reported: 1-7 days
Specimen Required: Collect: Buccal mucosa, eye, genital, rectal, throat or vesicle swab, or bronchoalveolar lavage, tissue or vesicle fluid.

Specimen Preparation: Fluid: Transfer to a sterile container. Also acceptable: Transfer to viral transport media (ARUP Supply #12884). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Swab or Tissue
: Place in viral transport media.
Place each specimen in an individually sealed bag.


Storage/Transport Temperature: Refrigerated.

Remarks: Specimen source preferred.

Unacceptable Conditions: Blood, CSF, plasma, or serum. Calcium alginate, dry, or wood swabs.

Stability (collection to initiation of testing): Ambient: 2 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Reference Interval:
Culture negative for herpes simplex virus.
Note: If culture is positive for HSV, then HSV typing test will be added. Additional charges apply.

Herpes Simplex Virus by PCR (ARUP test code 0060041) on CSF specimens is standard of care for diagnosing HSV meningitis/meningoencephalitis.
CPT Code(s): 87252; 87253; if reflexed, add 87140 x2