ARUP's Laboratory Test Directory

Antimicrobial Susceptibility - Extended Spectrum Beta Lactamase : 0063999

Mnemonic: MA ESBL

Methodology: Disk Diffusion
Performed: Sun-Sat
Reported: 1-2 days
Specimen Required: Collect: Actively growing E. coli, Klebsiella species, or Proteus mirabilis in pure culture.

Specimen Preparation: Transport sealed container with pure culture on agar slant or in bacterial transport media.

Storage/Transport Temperature: Room temperature.

Submit specimen according to Biological Substance, Category B, shipping guidelines.


Remarks: Isolate identification and specimen source required. Proteus mirabilis is tested only when isolated from a significant source (e.g., blood).

Unacceptable Conditions: Mixed cultures or non-viable organisms.

Stability (collection to initiation of testing): Ambient: 1 week; Refrigerated: 48 hours; Frozen: Unacceptable
Reference Interval:
Negative
Interpretive Data: Currently there are no CLSI guidelines for testing organisms other than E. coli, Klebsiella spp., and Proteus mirabilis for the presence of ESBL activity.  The CLSI-approved method does not rule out resistance by other mechanisms.
Note: The following agents are tested with and without clavulanic acid: ceftazidime and cefotaxime.

An additional handling fee will be billed for all organisms submitted that are not in pure culture as indicated in the specimen requirements.
CPT Code(s): 87184
Cross References: Extended Spectrum Beta Lactamase Susceptibility (Antimicrobial Susceptibility - Extended Spectrum Beta Lactamase)
 
 

 

 

 
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