ARUP's Laboratory Test Directory
| 0060738: AFB Culture (Includes AFB Stain 0060151) with Reflex to Mycobacterium tuberculosis Amplified Direct Detection (0060095) |
| Test Mnemonic: MC AFBR | |
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Methodology: Standard reference procedures for stain and culture. Identification of AFB is ordered and billed separately. DNA probes are available for M. tuberculosis complex and M. avium-intracellulare complex as indicated. DNA sequencing and other molecular techniques are used for identification. For drug susceptibilities, refer to Antimicrobial Susceptibility - AFB Mycobacteria (0060217). For AFB stain positive respiratory specimens, Mycobacterium tuberculosis Amplified Direct Detection (0060095) will be added at an additional charge.
Performed: Sun-Sat Reported: 1-56 days Stain: 24 hours; Final negative at 8 weeks Positive cultures are reported as soon as detected |
| Specimen Required: | |
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Collect: 10-15 mL sputum. Source of specimen is required.
Transport: Specimen in a sterile, leak-proof container at 2-8°C. Place each specimen in an individual sealed bag. Submit specimen according to Biological Substance, Category B, shipping guidelines. Pediatric Collection/Transport: Submit specimen according to Biological Substance, Category B, shipping guidelines. Remarks: Collection of early-morning specimens of sputum on each of three consecutive days is optimum. Indicate if mycobacteria other than tuberculosis (MOTT) are suspected. This may expedite identification. Clients will be notified of positive stain/culture. Unacceptable Conditions: Multiple same-site specimens (more than one in 24 hours), dry material, nonsterile or leaking containers, improper volume of sputum (less than 2 mL) or fluids (less than 1 mL), or material collected and transported on a SWAB. Stability: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 1 week |
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| Reference Interval: |
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Negative for acid-fast bacilli. Identification ordered and performed on positives. Susceptibility performed on all initial isolates of M. tuberculosis complex. Susceptibility performed on Mycobacterium other than M. tuberculosis complex isolates by request only. Susceptibility testing of M. gordonae is inappropriate. |
| Note: | |
| AFB stain, AFB identification of positives, and drug susceptibility tests are billed separately from culture. Identification of positive culture is billed by individual DNA probes and tests performed. Mycobacterium tuberculosis Amplified Direct Detection (0060095) will be performed on AFB stain positive specimens. |
| CPT Code(s): | |
| 87116 Culture. CPT codes for identification and susceptibility vary based on method. If reflexed, add 87556 Amplified probe. |