ARUP's Laboratory Test Directory

Respiratory Viruses DFA with Reflex to Viral Culture, Respiratory : 0060281

Mnemonic: RSPFAC

Ordering Recommendation: Order this test when the detection of respiratory viruses in addition to influenza is important. Test detects influenza (A, B), parainfluenza (1, 2, 3), adenovirus, respiratory syncytial virus (RSV), and human metapneumovirus (hMPV). Molecular methods are preferred for immunocompromised and hospitalized patients. For general inpatient workup of influenza-like illness, order the Respiratory Virus Mini Panel by RT-PCR (0060764).
Methodology: Direct Fluorescent Antibody Stain
Performed: Sun-Sat
Reported: Within 24 hours
Specimen Required: Collect: Respiratory specimen: Bronchoalveolar lavage (BAL), nasopharyngeal aspirate, swab, or washing, or tracheal aspirate.

Specimen Preparation: Fluid: Transfer specimen to a sterile container. Also acceptable: Transfer to viral transport media (ARUP supply #12884). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Swab
: Place in viral transport media.
Place each specimen in a separate, individually sealed bag.


Storage/Transport Temperature: Refrigerated.

Remarks: Specimen source preferred.

Unacceptable Conditions: Calcium alginate, dry or wood swabs. Slides.

Stability (collection to initiation of testing): Ambient: 2 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Reference Interval:
Negative
Note: If DFA is negative or inadequate, then a respiratory viral culture will be added. Additional charges apply.

Sensitivity of DFA methodology is dependent upon adequacy of the specimen.  If specimen contains fewer than 20 cells, then the DFA result will be reported as "inadequate." 
CPT Code(s): 87276; 87275; 87279 x3; 87280; 87260; 87299; if reflexed add 87252; if definitive identification performed, add 87253.