| Ordering Recommendation: |
Alternate test for detection of herpes simplex virus infection. Negative DFA result reflexes to viral culture for confirmation. Molecular testing is generally preferred.
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| Methodology: |
Direct Fluorescent Antibody Stain
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| Performed: |
Sun-Sat
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| Reported: |
Within 24 hours
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| Specimen Required: |
Collect: Buccal mucosa lesion, cervical or genital lesion, eye, throat or vesicle swab, bronchoalveolar lavage (BAL), tissue or vesicle fluid.
Specimen Preparation: Fluid: Transfer specimen to a sterile container. Also acceptable: Transfer to viral transport media (ARUP Supply #12884). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Swab or Tissue: Place in viral transport media. Place each specimen in an individually sealed bag.
Storage/Transport Temperature: Refrigerated.
Remarks: Specimen source preferred.
Unacceptable Conditions: Bone marrow, CSF, or whole blood. Calcium alginate, dry, or wood swabs.
Stability (collection to initiation of testing): Ambient: 4 hours; Refrigerated: 72 hours; Frozen: Unacceptable
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