ARUP's Laboratory Test Directory

Mycoplasma pneumoniae by PCR : 0060256

Mnemonic: MPNEUMOPCR

Methodology: Polymerase Chain Reaction
Performed: Mon, Wed, Fri
Reported: 1-4 days
Specimen Required: Collect:  Respiratory specimen: sputum, lung washes, tracheal aspirates, nasopharyngeal swab, pleural fluid, bronchoalveolar lavage (BAL), bronchial brushings, or CSF. Specimen source is required.

Transport:  2 mL respiratory specimen in sterile container or in viral transport media (Microtest M4) or 1 mL CSF in sterile container, frozen.

Submit specimen according to Biological Substance, Category B, shipping guidelines.


Pediatric Collection/Transport:  1 mL respiratory specimen in sterile container or in viral transport media (Microtest M4) or CSF in sterile container, frozen. (Less volume may decrease sensitivity of assay.)

Submit specimen according to Biological Substance, Category B, shipping guidelines.


Remarks:  Separate samples must be submitted when multiple tests are ordered.  Avoid repeated freeze/thaw cycles.

Unacceptable Conditions:  Nonsterile or leaking containers.  Respiratory aspirates in collection containers with tubing.  Samples tend to leak from these containers, compromising the specimen.

Stability:  Ambient: 8 hours; Refrigerated: 3 days; Frozen: 1 year
Reference Interval:
Negative: Mycoplasma pneumoniae DNA not detected by PCR.
Positive: Mycoplasma pneumoniae DNA detected by PCR.
Interpretive Data: A negative result does not rule out the presence of PCR inhibitors in the patient specimen, or Mycoplasma pneumoniae DNA concentrations below the level of detection of the assay.

This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory.
CPT Code(s): 87581
 
 

 

 

 
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