| Methodology: |
Culture
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| Performed: |
Sun-Sat
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| Reported: |
Within 42 days
Positives are reported as soon as detected.
Final: Negative at 6 weeks
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| Specimen Required: |
Patient Prep: CSF or blood should be collected within first week of illness; urine after the first week of illness. Blood is collected per blood culture procedure.
Collect: Green (sodium heparin) or sodium oxalate. Also acceptable: Bactec aerobic resin bottle or other commercial blood culture bottles, CSF, or urine.
Specimen Preparation: Blood: Transport 1 mL whole blood in heparin, sodium oxalate tube, or blood culture bottles. (Min: 1 mL)
CSF: Transport 0.5 mL CSF in a sterile container. (Min: 0.1 mL)
Urine: Dilute 1 mL urine 1:10 in 1 percent bovine serum albumin within one hour of collection. Transport 1 mL diluted urine in a sterile container. (Min: 0.1 mL)
Storage/Transport Temperature: Room temperature.
Remarks: Specimen source required. Leptospiras spp. may not survive extended transport time or in acidic urine.
Unacceptable Conditions: Clotted whole blood or whole blood submitted in anticoagulant other than those listed above. Urine not diluted with bovine serum albumin within 1 hour of collection or urine in preservative.
Stability (collection to initiation of testing): Blood culture bottles: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Other: Ambient 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
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