| Methodology: |
Standard reference procedures for Leptospira bacterial culture and exam
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| Performed: |
Sun-Sat
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| Reported: |
Within 42 days
Positives are reported as soon as detected.
Final: Negative at 6 weeks
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| Specimen Required: |
Collect: Whole blood or CSF (week 1 of disease), urine (beyond 1 week of disease). Testing performed for University of Utah Health Sciences Center and local Utah clients only. Source of specimen is required.
Transport: CSF or urine in sterile container. Whole blood in EDTA, SPS, heparin, sodium oxalate tube, or in BACTE® or other commercial blood culture bottles. Deliver to the lab immediately.
Submit specimen according to Biological Substance, Category B, shipping guidelines.
Pediatric Collection/Transport:
Submit specimen according to Biological Substance, Category B, shipping guidelines.
Remarks: Leptospiras may not survive acid urine for more than a few hours. Media is available from the Microbiology Laboratory for direct inoculation.
Unacceptable Conditions: Clotted whole blood, nonsterile or leaking containers, cold or frozen specimens. Whole blood submitted in anticoagulant other than those listed above. Urine greater than 2 hours from collection or in preservative.
Stability: Ambient: CSF & urine - 2 hours; Whole blood - 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
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