| Methodology: |
Standard reference procedures for Leptospira bacterial culture and exam
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| Performed: |
Sun-Sat
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| Reported: |
Within 42 days
Positives are reported as soon as detected.
Final: Negative at 6 weeks
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| Specimen Required: |
Collect: Testing performed for UUHC and local Utah clients only.
Whole blood or CSF (week 1 of disease). OR urine (beyond week 1 of disease).
Specimen Preparation: Transport 7 mL whole blood in SPS, heparin or sodium oxalate tube, BACTEC® or other commercial blood culture bottles. (Min: 1 mL)
OR transport 0.5 mL CSF in sterile container. (Min: 0.1 mL)
OR transport 1 mL urine diluted 1:10 in 1% bovine serum albumin. (Min: 0.1 mL)
Specimen must be diluted 1:10 with 1% bovine serum albumin within 1 hour of collection and prior to transport to Bacteriology.
Storage/Transport Temperature: Room temperature. Deliver to Bacteriology immediately.
Submit specimen according to Biological Substance, Category B, shipping guidelines.
Remarks: Specimen source required. Leptospiras may not survive in acidic urine.
Unacceptable Conditions: Clotted whole blood or whole blood submitted in anticoagulant other than those listed above. Urine not diluted with bovine serum albumin within 1 hour of collection or urine in preservative. Frozen or refrigerated specimens.
Stability (collection to initiation of testing): Ambient: Heparinized or oxalated whole blood, CSF, urine diluted with bovine serum albumin: 24 hours; Whole blood in SPS or blood culture bottle: 6 days; Refrigerated: Unacceptable; Frozen: Unacceptable
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