Leptospira Culture : ARUP Lab Tests

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0060158: Leptospira Culture

Time Sensitive

UUHSC Testing Only

Test Mnemonic: MC LEPTO
Methodology: Standard reference procedures for Leptospira bacterial culture and exam Performed: Sun-Sat Reported: Within 42 days Positives are reported as soon as detected. Final: Negative at 6 weeks

Specimen Required:

Collect: Whole blood or CSF (week 1 of disease), urine (beyond 1 week of disease). Testing performed for University of Utah Health Sciences Center and local Utah clients only. Source of specimen is required.

Transport: CSF or urine in sterile container. Whole blood in EDTA, SPS, heparin, sodium oxalate tube, or in BACTE® or other commercial blood culture bottles. Deliver to the lab immediately.

Submit specimen according to Biological Substance, Category B, shipping guidelines.

Pediatric Collection/Transport:

Submit specimen according to Biological Substance, Category B, shipping guidelines.

Remarks: Leptospiras may not survive acid urine for more than a few hours. Media is available from the Microbiology Laboratory for direct inoculation.

Unacceptable Conditions: Clotted whole blood, nonsterile or leaking containers, cold or frozen specimens. Whole blood submitted in anticoagulant other than those listed above. Urine greater than 2 hours from collection or in preservative.

Stability: Ambient: CSF & urine - 2 hours; Whole blood - 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval:

Culture negative for Leptospira.

CPT Code(s):
87081 Culture, presumptive; 87166 Darkfield exam without collection

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