ARUP's Laboratory Test Directory

Immune Complex Panel : 0050667

Mnemonic: C1Q/RAJI

Methodology: Quantitative Flow Cytometry/Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Performed: Tue
Reported: 2-9 days
Specimen Required: Collect: Plain red.

Specimen Preparation: Let stand for 2 hours, then separate serum from cells within 1 hour. Transfer TWO 1.5 mL aliquots of serum to individual ARUP Standard Transport Tubes and freeze immediately. (Min: 0.5 mL/aliquot)

Storage/Transport Temperature: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions: Non-frozen specimens. Specimens exposed to repeated freeze/thaw cycles.

Stability (collection to initiation of testing): Refer to individual components.
Reference Interval:
Available Separately Components Reference Interval
0050301C1q Binding Assay Less than 4 µgE/mL is considered negative for circulating complement binding immune complexes.
0050302Raji Cell Immune Complex Assay By report

Interpretive Data: Many autoimmune disorders, chronic infections and malignancies are associated with circulating immune complexes. Quantitation of immune complexes assists in staging immunologic disorders.

Refer to Statement A under Testing Information at http://www.aruplab.com.
Note: Immune complexes bind to Raji Cells via their complement receptors.  The immunoglobulin portion of the immune complex is then detected by flow cytometry with anti-human IgG-FITC.
CPT Code(s): 86332 x2
Cross References: Circulating Immune Complex Panel (Immune Complex Panel), Detection of C3d Circulating Immune Complexes (Immune Complex Panel)