ARUP's Laboratory Test Directory
| 0050317: Anti-Nuclear Antibody (ANA), IgG Screen with Reflex to ANA IFA Titer, dsDNA, RNP, Smith, SSA, & SSB Antibodies |
| Test Mnemonic: ANA REF | |
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#ExistMethodology>
Methodology: Enzyme-Linked Immunosorbent Assay/Indirect Fluorescent Antibody/Multi-Analyte Fluorescent Detection
*ExistMethodology> #ExistPerformed> Performed: Sun-Sat *ExistPerformed> #ExistReported> Reported: 1-5 days *ExistReported> |
| Specimen Required: | |
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#ExistCollect>
Collect: One 4 mL SST.
*ExistCollect> #ExistTransport> Transport: 2 mL serum at 2-8°C. (Min: 1 mL) Submit specimen in an ARUP Standard Transport Tube. *ExistTransport> #ExistPedCollectTransport> Pediatric Collection/Transport: 0.5 mL serum at 2-8°C. *ExistPedCollectTransport> #ExistRemarks> Remarks: Separate serum from cells ASAP. Avoid repeated freeze/thaw cycles. *ExistRemarks> #ExistConditions> Unacceptable Conditions: Plasma. Severely lipemic, contaminated, or hemolyzed specimens. *ExistConditions> #ExistStability> Stability: After separation from cells: Ambient: 2 days; Refrigerated: 2 weeks; Frozen: 1 year *ExistStability> |
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| Reference Interval: |
| #ExistRefRange> By report *ExistRefRange> |
| Note: | |
| Specimens are screened for ANA using ELISA. If the ELISA screen is positive, then an IFA titer using HEp-2 substrate will be added. If confirmed by IFA, then a titer and pattern will be reported and the specimens will be tested for dsDNA antibody and ENA (RNP, Smith, SSA, and SSB) antibodies. |
| CPT Code(s): | |
| 86038 ANA; if reflexed, add 86039 ANA titer + 86235 x4 ENAs + 86225 dsDNA |