ARUP's Laboratory Test Directory

0050317: Anti-Nuclear Antibody (ANA), IgG Screen with Reflex to ANA IFA Titer, dsDNA, RNP, Smith, SSA, & SSB Antibodies

Test Mnemonic: ANA REF
Methodology: Enzyme-Linked Immunosorbent Assay/Indirect Fluorescent Antibody/Multi-Analyte Fluorescent Detection

Performed: Sun-Sat

Reported: 1-5 days

Specimen Required:  
Collect: One 4 mL SST. 

Transport: 2 mL serum at 2-8°C.  (Min: 1 mL)  Submit specimen in an ARUP Standard Transport Tube.

Pediatric Collection/Transport: 0.5 mL serum at 2-8°C.

Remarks: Separate serum from cells ASAP.  Avoid repeated freeze/thaw cycles.

Unacceptable Conditions: Plasma.  Severely lipemic, contaminated, or hemolyzed specimens.

Stability: After separation from cells: Ambient: 2 days; Refrigerated: 2 weeks; Frozen: 1 year

Reference Interval:
By report

Note:
Specimens are screened for ANA using ELISA.  If the ELISA screen is positive, then an IFA titer using HEp-2 substrate will be added.  If confirmed by IFA, then a titer and pattern will be reported and the specimens will be tested for dsDNA antibody and ENA (RNP, Smith, SSA, and SSB) antibodies.

CPT Code(s):
86038 ANA; if reflexed, add 86039 ANA titer + 86235 x4 ENAs + 86225 dsDNA