Anti-Nuclear Antibody (ANA), IgG Screen with Reflex to ANA IFA Titer, dsDNA, RNP, Smith, SSA, & SSB Antibodies : ARUP Lab Tests

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Anti-Nuclear Antibody (ANA), IgG Screen with Reflex to ANA IFA Titer, dsDNA, RNP, Smith, SSA, & SSB Antibodies : 0050317

Mnemonic: ANA REF

Methodology:	Enzyme-Linked Immunosorbent Assay/Indirect Fluorescent Antibody/Multi-Analyte Fluorescent Detection
Performed:	Sun-Sat
Reported:	1-5 days
Specimen Required:	Collect: One 4 mL serum separator tube. Transport: 2 mL serum at 2-8°C. (Min: 1 mL) Submit specimen in an ARUP Standard Transport Tube. Pediatric Collection/Transport: 0.5 mL serum at 2-8°C. Remarks: Separate serum from cells ASAP. Avoid repeated freeze/thaw cycles. Unacceptable Conditions: Plasma. Severely lipemic, contaminated, or hemolyzed specimens. Stability: After separation from cells: Ambient: 2 days; Refrigerated: 2 weeks; Frozen: 1 year

Reference Interval: By report
Note:	Specimens are screened for ANA using ELISA. If the ELISA screen is positive, then an IFA titer using HEp-2 substrate will be added. If confirmed by IFA, then a titer and pattern will be reported and the specimens will be tested for dsDNA antibody and ENA (RNP, Smith, SSA, and SSB) antibodies.
CPT Code(s):	86038 ANA; if reflexed, add 86039 ANA titer + 86235 x4 ENAs + 86225 dsDNA

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