ARUP's Laboratory Test Directory
| 0050268: Borrelia burgdorferi Total Antibodies, IgG and/or IgM by ELISA with Reflex to IgG by Western Blot (Late Disease) |
| Test Mnemonic: LYME CHRO | |
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Methodology: Enzyme-Linked Immunosorbent Assay/Western Blot
Performed: Sun-Sat Reported: 1-3 days |
| Specimen Required: | |
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Collect: One 4 mL SST.
Transport: 2 mL serum at 2-8°C. (Min: 0.2 mL) Submit specimen in an ARUP Standard Transport Tube. Pediatric Collection/Transport: 0.1 mL serum at 2-8°C. Remarks: Separate serum from cells ASAP. Unacceptable Conditions: CSF or plasma. Severely lipemic, heat-inactivated, contaminated, or hemolyzed specimens. Stability: After separation from cells: Ambient: 2 days; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) |
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| Note: | |
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This panel is appropriate for Lyme disease testing greater than four weeks from erythema migrans or onset of disease symptoms. A negative result indicates that the Western blot evaluation for Borrelia burgdorferi antibody demonstrates no antibodies unique to Borrelia burgdorferi, and therefore is not supportive of Lyme disease. A positive result indicates that the Western blot evaluation for Borrelia burgdorferi antibody is consistent with the presence of antibody produced by patients in response to infection by Borrelia burgdorferi and suggests the presence of Lyme disease. Although the test has been shown to have a high degree of reliability for diagnostic purposes, laboratory data should always be correlated with clinical findings. Current CDC recommendations for the serological diagnosis of Lyme disease are to screen with a polyvalent ELISA test and confirm equivocals and positives with Western blot. Both IgM and IgG Western blots should be performed on samples obtained less than four weeks after appearance of erythema migrans. Only IgG Western blot is to be performed on samples greater than four weeks after disease onset. IgM Western blot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease. If ELISA result is 1.00 LIV, then IgG Western blot will be added. |
| CPT Code(s): | |
| 86618 B. burgdorferi total Ab. IgG/IgM; if reflexed, add 86617 Borrelia burgdorferi Western blot IgG |