ARUP's Laboratory Test Directory

Entamoeba histolytica (amebiasis), Antibody, IgG : 0050070

Mnemonic: AMB

Methodology: Enzyme-Linked Immunosorbent Assay
Performed: Tue, Fri
Reported: 1-5 days
Specimen Required: Collect:  One 4 mL serum separator tube.

Transport:  1 mL serum at 2-8°C. (Min: 0.5 mL)  Submit specimen in an ARUP Standard Transport Tube.

Pediatric Collection/Transport:  0.15 mL serum at 2-8°C.

Remarks:  Separate serum from cells ASAP.  Acute and convalescent specimens must be labeled as such; parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens.  Please mark specimen plainly as "acute" or "convalescent." 

Unacceptable Conditions:  Severely lipemic, contaminated, heat-inactivated, or hemolyzed specimens.

Stability:  After separation from cells: Ambient: 2 days; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval:
0.79 IV or less: Negative - No significant level of detectable E. histolytica IgG antibody.
0.80-1.19 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.20 IV or greater: Positive - IgG antibody to E. histolytica detected, suggestive of a current or past infection.
Interpretive Data: Seroconversion between acute and convalescent sera is considered strong evidence of recent infection.  The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time.
Note: In the case of extraintestinal complications, a positive antibody can indicate amebiasis even though stool findings are negative.
CPT Code(s): 86753
 
 

 

 

 
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