ARUP's Laboratory Test Directory

0050070: Entamoeba histolytica (amebiasis), Antibody, IgG

Test Mnemonic: AMB
Methodology: Enzyme-Linked Immunosorbent Assay

Performed: Tue, Fri

Reported: 1-5 days

Specimen Required:  
Collect: One 4 mL SST.

Transport: 1 mL serum at 2-8°C. (Min: 0.5 mL)  Submit specimen in an ARUP Standard Transport Tube.

Pediatric Collection/Transport: 0.15 mL serum at 2-8°C.

Remarks: Separate serum from cells ASAP.  Acute and convalescent specimens must be labeled as such; parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens.  Please mark specimen plainly as "acute" or "convalescent." 

Unacceptable Conditions: Severely lipemic, contaminated, heat-inactivated, or hemolyzed specimens.

Stability: After separation from cells: Ambient: 2 days; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval:
0.79 IV or less: Negative - No significant level of detectable E. histolytica IgG antibody.
0.80-1.19 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.20 IV or greater: Positive - IgG antibody to E. histolytica detected, suggestive of a current or past infection.

Interpretive Data:
Seroconversion between acute and convalescent sera is considered strong evidence of recent infection.  The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time.



Note:
In the case of extraintestinal complications, a positive antibody can indicate amebiasis even though stool findings are negative.

CPT Code(s):
86753

 

 

 
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