ARUP's Laboratory Test Directory

p53 Tissue Assay, Paraffin : 0049250

Mnemonic: P53 IP

Methodology: Immunohistochemistry
Performed: Mon-Fri
Reported: 1-5 days
Specimen Required: Collect:  Tumor tissue.

Transport:  Paraffin-embedded, formalin-fixed tissue block enriched with tumor at 20-25°C. Protect paraffin block from excessive heat.  Ship in cooled container during summer months. Include surgical pathology report. 

Unacceptable Conditions:  Paraffin block with no tumor tissue remaining; samples fixed in  B-5 or Bouin's fixative.

Stability:  Paraffin block: Ambient: Indefinitely; Refrigerated: Acceptable; Frozen: Unacceptable
Reference Interval:
Negative - Good Prognosis (< 10% of nuclei stain)
Positive - Poor Prognosis (> 10% of nuclei stain)
Interpretive Data: Assay is performed on paraffin-embedded, formalin-fixed tissue.  Antibody clone is DO-7 and the detection system is LSAB. Although many studies have shown an association between p53 staining and poor prognosis, some articles have shown no association. This association is currently an area of active investigation. This test uses a commercial kit or reagent that has not been approved or cleared by the FDA.  Its performance characteristics were determined by ARUP Laboratories. Results of this test should be interpreted with caution in the context of other data.
CPT Code(s): 88360
 
 

 

 

 
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