ARUP's Laboratory Test Directory

Thrombotic Risk, Inherited Etiologies (Most Common) with Reflex to Factor V Leiden : 0030133

Mnemonic: THROM COM

Ordering Recommendation: Acceptable panel to screen for common inherited thrombophilias.
Methodology: Electromagnetic Clot Detection/Quantitative Enzymatic/Polymerase Chain Reaction/Fluorescence Monitoring
Performed: Refer to individual components
Reported: 2-7 days
Specimen Required: Patient Prep: Fasting specimen preferred for homcysteine (green, sodium or lithium heparin).

Collect: Lt. blue (sodium citrate) AND lavender (EDTA) or pink (K2EDTA) AND green (sodium or lithium heparin).

Specimen Preparation: Transport two 2 mL aliquots platelet-poor plasma (sodium citrate) (Min: 1 mL/aliquot) AND 1 mL plasma (sodium or lithium heparin) (Min: 0.4 mL) AND 5 mL whole blood (EDTA).

Storage/Transport Temperature: Platelet-poor Plasma: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Plasma: Frozen.
Whole Blood: Refrigerated.

Unacceptable Conditions: Refer to individual components.

Stability (collection to initiation of testing): Refer to individual components.
Reference Interval:
 
Test Number Components Reference Interval
0030235Partial Thromboplastin Time 24-35 seconds
0030095Factor VIII, Activity 0-6 years: 56-191%
7-9 years: 76-199%
10-11 years: 80-209%
12-13 years: 72-198%
14-15 years: 69-237%
16-17 years: 63-221%
18 years and older: 56-191%
0099869Homocysteine, Total < 11 µmol/L, for both male and female
0056060Prothrombin (F2) G20210A Mutation Negative: This sample is negative for the factor II, prothrombin 20210A mutation.
 APC Resistance Profile Effective February 21, 2011

2.00 or greater
 
Test Number Components Reference Interval
0030127APC Resistance Profile Refer to report
0097720Factor V Leiden (F5) R506Q Mutation Refer to report
 Factor V Leiden by PCR & Fluorescence Monitoring Negative: The sample is negative for factor V Leiden, R506Q mutation.
 
Interpretive Data: Ratios less than 2.00 suggest APC resistance. This method uses factor V deficient plasma; therefore, APC resistance due to a nonfactor V mutation will not be detected. Extreme factor V deficiency may cause abnormal ratio.
Note: If APC resistance is normal, then no further testing will be added. If APC resistance is abnormally low, then Factor V Leiden by PCR will be added. Additional charges apply.
CPT Code(s): 85730, 85240, 83090, 81240, 85307; If reflexed, add 81241
Cross References: Hypercoag (Thrombotic Risk, Inherited Etiologies (Most Common) with Reflex to Factor V Leiden), Hypercoagulable Panel (Thrombotic Risk, Inherited Etiologies (Most Common) with Reflex to Factor V Leiden) , Thrombophilia (Thrombotic Risk, Inherited Etiologies (Most Common) with Reflex to Factor V Leiden)