ARUP's Laboratory Test Directory

Alkaline Phosphatase Isoenzymes, Serum or Plasma : 0021020

Mnemonic: ALKP-ISO

Methodology: Quantitative Heat Inactivation/Enzymatic
Performed: Sun-Sat
Reported: 1-3 days
Specimen Required: Collect: Serum separator tube or green (sodium or lithium heparin).

Specimen Preparation: Allow serum specimen to clot completely at room temperature. Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube and refrigerate or freeze immediately. (Min: 1 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Specimens collected in EDTA, sodium fluoride, or potassium oxalate. Grossly hemolyzed or lipemic specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 1 hour; Refrigerated: 1 week (total activity will increase 2 percent per day); Frozen: 1 year
Reference Interval:
 
Test Number Components   
0020005 Alkaline Phosphatase, Serum or Plasma Male
0-30 days: 60-320 U/L
1-11 months: 70-350 U/L
1-3 years: 125-320 U/L
4-6 years: 150-370 U/L
7-9 years: 150-440 U/L
10-11 years: 150-470 U/L
12-13 years: 160-500 U/L
14-15 years: 130-530 U/L
16-19 years: 60-270 U/L
20 years and older: 40-120 U/L 		Female
0-30 days: 60-320 U/L
1-11 months: 70-350 U/L
1-3 years: 125-320 U/L
4-6 years: 150-370 U/L
7-9 years: 150-440 U/L
10-11 years: 150-530 U/L
12-13 years: 110-525 U/L
14-15 years: 55-305 U/L
16-19 years: 40-120 U/L
20 years and older: 40-120 U/L
 Bone Male
1-6 years: 0-208 U/L
7-9 years: 0-264 U/L
10-15 years: 0-340 U/L
16-19 years: 0-165 U/L
20 years and older: 0-55 U/L 		Female
1-6 years: 0-189 U/L
7-9 years: 0-246 U/L
10-13 years: 0-340 U/L
14-15 years: 0-91 U/L
16 years and older: 0-55 U/L
 Liver Male
1-6 years: 0-145 U/L
7-11 years: 0-182 U/L
12-15 years: 0-226 U/L
16-19 years: 0-114 U/L
20 years and older: 0-94 U/L 		Female
1-9 years: 0-148 U/L
10-15 years: 0-162 U/L
16 years and older: 0-94 U/L

Interpretive Data: Bone Specific Alkaline Phosphatase (0070053) and 5'Nucleotidase (0080235) may be useful in identifying disorders of bone and liver, respectively.
Note: Heat-stable isoenzymes, such as the one produced during pregnancy, will interfere with the interpretation of this assay.
CPT Code(s): 84075; 84080
Cross References: Alkaline Phosphatase Isoenzymes by Heat Fractionation (Alkaline Phosphatase Isoenzymes)