| Methodology: |
Refer to individual components
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| Performed: |
Mon-Fri
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| Reported: |
Refer to individual components
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| Specimen Required: |
Collect: 24-hour urine. Specimen should be refrigerated during collection.
Transport: 1st aliquot: No adjustment to pH. Transport 4 mL aliquot at 2-8°C. (Min: 0.5 mL) Submit specimen in an ARUP Standard Transport Tube. Record total volume, collection time interval, and "no adjustment to pH" on transport tube and test request form.
2nd aliquot: Adjust entire 24-hour collection to a pH less than or equal to 2 by adding 6M HCl (approximately 10 mL HCl/24-hour specimen based on normal adult output of 1000-2000 mL/24 hours. Pediatric specimens will require < 10 mL to reach the correct pH). Mix well. Transport 4 mL aliquot at 2-8°C. (Min: 0.5 mL) Submit specimen in an ARUP Standard Transport Tube. Record 24-hour total volume, collection time interval, and pH on transport tube and test request form.
3rd aliquot: Adjust the remaining 24-hour collection to a pH greater than or equal to 8 by adding 6M NaOH (approximately 20 mL 6M NaOH/24-hour specimen based on normal adult output of 1000-2000 mL/24 hours. Pediatric specimens will require < 20 mL to reach the correct pH). Mix well and allow the entire 24-hour collection to stand for 15 minutes. Transport 4 mL aliquot at 2-8°C. (Min: 0.5 mL) Submit specimen in an ARUP Standard Transport Tube. Record 24-hour total volume, collection time interval, and pH on transport tube and test request form.
Stability: Ambient: 8 hours; Refrigerated: 4 days; Frozen: 3 weeks
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