Welcome to ARUP Immunology
What We Do Best
Molecular ImmunologyA collection of molecular tests to screen, diagnose, or prognose immunological diseases, conditions, and pharmacological response.
- Find an HLA test (search list)
- Transplant compatibility:
- High-resolution HLA genotyping by NGS
2012482 HLA-A by Next Generation Sequencing 2012486 HLA-B by Next Generation Sequencing 2012490 HLA-C by Next Generation Sequencing 2012494 HLA-DRB1 by Next Generation Sequencing 2012498 HLA-DQB1 by Next Generation Sequencing 2012502 HLA-DPB1 by Next Generation Sequencing
ARUP began offering high-resolution HLA genotyping by next-generation sequencing (NGS) for two HLA tests, HLA Class I (A, B, and C) and HLA Class II (DRB1 and DQB1) under an agreement with The Children’s Hospital of Philadelphia.
Currently, the standard of care for HLA genotyping to select the best matched donor for hematopoietic stem cell transplantation (HSCT) is to use a low/intermediate resolution SSO/SSP test first, followed by high-resolution Sanger sequencing. HLA typing by NGS generates the high-resolution allelic information in patients and donor candidates in a single step without the need for screening and progression to confirmatory testing.
HLA genes are the most polymorphic gene family found in the human genome, with more than 10,000 different HLA alleles reported to date. High-resolution HLA genotyping by NGS is uniquely able to address limitations of traditional Sanger sequencing assays in patients requiring a higher level of HLA allele matching.
Sanger Sequencing NGS NGS HLA genotyping by Sanger sequencing is derived from few exons of HLA class I and class II genes. HLA genotyping by NGS is derived from all exons of HLA class I and class II genes. Sanger sequencing fails to solve cis/trans chromosomal polymorphic positions of HLA alleles NGS is able to solve cis/trans chromosomal polymorphic positions of HLA alleles. Sanger sequencing produces ambiguous results, with need for National Marrow Donor Program (NMDP) coding. NGS produces unambiguous results, without need for National Marrow Donor Program (NMDP) coding.
- Drug therapy
2002429 HLA-B*57:01 for Abacavir Sensitivity 2012049 2012049 HLA-B*15:02 Genotyping, Carbamazepine Hypersensitivity
2005023 Narcolepsy (HLA-DQB1*06:02) Genotyping 0050392 Ankylosing Spondylitis (HLA-B27) Genotyping 2005018 Celiac Disease (HLA-DQ2, and HLA-DQ8) Genotyping 2006984 HLA-A Genotype 2006986 HLA-B Genotype 2006988 HLA-C Genotype 2002798 HLA-DR Genotyping 2014073 HLA-DP Genotyping 2014079 HLA-DQ Genotyping 0051067 HLA DRB 3*,4*,5*
Heparin Dependent Antibody
The Immunology services at ARUP offer a wide range of testing;
- The Immunology Core section performs highly automated, high-volume assays with rapid throughput. Methodology includes enzyme-linked immunosorbent assay and chemiluminescent assay.
- Cellular and Innate lab perform highly esoteric assays that test functional components of the cell-mediated and innate branches of the immune system.
- Microbial Immunology lab performs assays to aid in the serologic diagnosis of bacterial, viral, mycoplasma, and parasitic infections.
- Protein immunology lab employs a wide variety of methods to test for complement deficiencies, monoclonal and polyclonal gammopathies, and immunoglobulin deficiencies.
- Immunologic Flow Cytometry lab uses laser detection of fluorescent-labeled antibodies to identify cells by their antigen expression.
- Serologic lab aids in the diagnosis of viral hepatitis, HIV.
- Autoimmune immunology performs assays to detect antibodies produced by the immune system as it attacks its own body. This area employs multi-analyte fluorescence detection, Ouchterlony double immunodiffusion, immunoblot, indirect fluorescent antibody methods, and ELISA.
The division is supported by R&D scientists who are focused on new test development. Medical directors are board certified in their areas of expertise. They are available to answer questions regarding test results and consult with clients on difficult to diagnose cases.
For test inquiries or to arrange a consultation, please contact ARUP Client Services at (800) 522-2787.