The Institute for Clinical and Experimental Research® is focused on the development of new diagnostic assays in a wide range of disciplines. Researchers at the institute regularly publish in respected, peer-reviewed journals, give presentations at national and international organizations, author books and book chapters, and develop new tests.

There are 99 total entries for the year 2005.

Little RR, Vesper H, Rohlfing CL, Ospina M, Safar-Pour S, Roberts WL. Validation by a mass spectrometric reference method of use of boronate affinity chromatography to measure glycohemoglobin in the presence of hemoglobin S and C traits. Clinical Chemistry 2005;51(1):264–5.

Layfield LJ, Cramer H. Fine-needle aspiration cytology of intraductal papillary-mucinous tumors: a retrospective analysis. Diagnostic Cytopathology 2005;32(1):16–20.

Lolekha PH, Chittamma A, Roberts WL, Sritara P, Cheepudomwit S, Suriyawongpaisal P. Comparative study of two automated high-sensitivity C-reactive protein methods in a large population. Clinical Biochemistry 2005;38(1):31–5.

Choi HH, Gray PB, Storer TW, Calof OM, Woodhouse L, Singh AB, Padero C, Mac RP, Sinha-Hikim I, Shen R, Dzekov J, Dzekov C, Kushnir MM, Rockwood AL, Me. Effects of testosterone replacement in human immunodeficiency virus-infected women with weight loss. Journal of Clinical Endoctrinology and Metabolism 2005;90(3):1531–41.

Joyner DE, Wade ML, Szabo A, Bastar J, Coffin CM, Albritton KH, Bernard PS, Randall RL. Discriminate gene lists derived from cDNA microarray profiles of limited samples permit distinguishing mesenchymal neoplasia ex vivo. Journal of Cancer Research and Clinical Oncology 2005;131(3):137–46.

Clarke JL, Anderson JL, Carlquist JF, Roberts RF, Horne BD, Bair TL, Kolek MJ, Mower CP, Crane AM, Roberts WL, Muhlestein JB. Comparison of differing C-reactive protein assay methods and their impact on cardiovascular risk assessment. American Journal of Cardiology 2005;95(1):155–8.

Seipp MT, Erali M, Wies RL, Wittwer C. HLA-B27 typing: evaluation of an allele-specific PCR melting assay and two flow cytometric antigen assays. Cytometry Part B- Clinical Cytometry 2005;63(1):10–5.

Kishore BK, Isaac J, Fausther M, Tripp SR, Shi H, Gill PS, Braun N, Zimmermann H, Sevigny J, Robson SC. Expression of NTPDase1 and NTPDase2 in murine kidney: relevance to regulation of P2 receptor signaling. American Journal of Physiology- Renal Physiology 2005;288(5):F1032–43.

Petti CA, Mirrett S, Woods CW, Reller LB. Controlled clinical comparison of plastic versus glass bottles of BacT/ALERT PF medium for culturing blood from children. Journal of Clinical Microbiology 2005;43(1):445–7.

Eliason K, Hendrickson BC, Judkins T, Norton M, Leclair B, Lyon E, Ward B, Noll W, Scholl T. The potential for increased clinical sensitivity in genetic testing for polyposis colorectal cancer through the analysis of MYH mutations in North American patients. Journal of Medical Genetics 2005;42(1):95–6.

Layfield LJ, Cramer H. Primary sclerosing cholangitis as a cause of false positive bile duct brushing cytology: report of two cases. Diagnostic Cytopathology 2005;32(2):119–24.

Gingeras TR, Higuchi R, Kricka LJ, Lo YM, Wittwer CT. Fifty years of molecular (DNA/RNA) diagnostics. Clinical Chemistry 2005;51(3):661–71.

Chou LS, Gedge F, Lyon E. Complete gene scanning by temperature gradient capillary electrophoresis using the cystic fibrosis transmembrane conductance regulator gene as a model. Journal of Molecular Diagnostics 2005;7(1):111–20.

Singh RJ, Grebe SK, Yue B, Rockwood AL, Cramer JC, Gombos Z, Eisenhofer G. Precisely wrong? Urinary fractionated metanephrines and peer-based laboratory proficiency testing. Clinical Chemistry 2005;51(2):472–3.

Roberts WL, Safar-Pour S, De BK, Rohlfing CL, Weykamp CW. Effects of hemoglobin C and S traits on glycohemoglobin measurements by eleven methods. Clinical Chemistry 2005;51(4):776–8.

Konnick EQ, Erali M, Ashwood ER, Hillyard DR. Evaluation of the COBAS amplicor HBV monitor assay and comparison with the ultrasensitive HBV hybrid capture 2 assay for quantification of hepatitis B virus DNA. Journal of Clinical Microbiology 2005;43(2):596–603.

Shakespeare WA, Davie D, Tonnerre C, Rubin MA, Strong M, Petti CA. Nalidixic acid-resistant Salmonella enterica serotype Typhi presenting as a primary psoas abscess: case report and review of the literature. Journal of Clinical Microbiology 2005;43(2):996–8.

Owen WE, Roberts WL. Performance characteristics of the IMMULITE 2000 insulin-like growth factor binding protein-3 assay. Clinica Chimica Acta 2005;353(1–2):141–5.

Dobrowolski SF, McKinney JT, Amat di San Filippo C, Giak Sim K, Wilcken B, Longo N. Validation of dye-binding/high-resolution thermal denaturation for the identification of mutations in the SLC22A5 gene. Human Mutation 2005;25(3):306–13.

Rawlins ML, Owen WE, Roberts WL. Performance characteristics of four automated natriuretic peptide assays. American Journal of Clinical Pathology 2005;123(3):439–45.

Crockett DK, Fillmore GC, Elenitoba-Johnson KS, Lim MS. Analysis of phosphatase and tensin homolog tumor suppressor interacting proteins by in vitro and in silico proteomics. Proteomics 2005;5(5):1250–62.

Aldous WK, Gerber K, Taggart EW, Rupp J, Wintch J, Daly JA. A comparison of Thermo Electron RSV OIA to viral culture and direct fluorescent assay testing for respiratory syncytial virus. Journal of Clinical Virology 2005;32(3):224–8.

Stinnett JM, Pendleton R, Skordos L, Wheeler M, Rodgers GM. Venous thromboembolism prophylaxis in medically ill patients and the development of strategies to improve prophylaxis rates. American Journal of Hematology 2005;78(3):167–72.

Miller WG, Myers GL, Ashwood ER, Killeen AA, Wang E, Thienpont LM, Siekmann L. Creatinine measurement: state of the art in accuracy and interlaboratory harmonization. Archives of Pathology and Laboratory Medicine 2005;129(3):297–304.

Bornhorst JA, Hunt JW, Urry FM, McMillin GA. Comparison of sample preservation methods for clinical trace element analysis by inductively coupled plasma mass spectrometry. American Journal of Clinical Pathology 2005;123(4):578–83.

Schwarz EL, Roberts WL, Pasquali M. Analysis of plasma amino acids by HPLC with photodiode array and fluorescence detection. Clin Chim Acta 2005;354(1–2):83–90.

Schwarz EL, Roberts WL, Pasquali M. Analysis of plasma amino acids by HPLC with photodiode array and fluorescence detection. Clinica Chimica Acta 2005;354(1–2):83–90.

Shutt CK, Pounder JI, Page SR, Schaecher BJ, Woods GL. Clinical evaluation of the DiversiLab microbial typing system using repetitive-sequence-based PCR for characterization of Staphylococcus aureus strains. Journal of Clinical Microbiology 2005;43(3):1187–92.

Balagopal P, George D, Patton N, Yarandi H, Roberts WL, Bayne E, Gidding S. Lifestyle-only intervention attenuates the inflammatory state associated with obesity: a randomized controlled study in adolescents. Journal of Pediatrics 2005;146(3):342–8.

Odell ID, Cloud JL, Seipp M, Wittwer CT. Rapid species identification within the Mycobacterium chelonae-abscessus group by high-resolution melting analysis of hsp65 PCR products. American Journal of Clinical Pathology 2005;123(1):96–101.

Cessna MH, Hartung L, Tripp S, Perkins SL, Bahler DW. Hairy cell leukemia variant: fact or fiction. American Journal of Clinical Pathology 2005;123(1):132–8.

Kushnir MM, Rockwood AL, Nelson GJ, Yue B, Urry FM. Assessing analytical specificity in quantitative analysis using tandem mass spectrometry. Clinical Biochemistry 2005;38(4):319–27.

Layfield LJ, Willmore-Payne C, Shimada H, Holden JA. Assessment of NMYC amplification: a comparison of FISH, quantitative PCR monoplexing and traditional blotting methods used with formalin-fixed, paraffin-embedded neuroblastomas. Analytical and Quantitative Cytology and Histology 2005;27(1):5–14.

Petti CA, Mirrett S, Woods CW, Reller LB. Controlled clinical comparison of plastic and glass bottles of BacT/ALERT FA medium for culturing organisms from blood of adult patients. Journal of Clinical Microbiology 2005;43(4):1960–2.

Willmore-Payne C, Layfield LJ, Holden JA. c-KIT mutation analysis for diagnosis of gastrointestinal stromal tumors in fine needle aspiration specimens. Cancer 2005;105(3):165–70.

Rockwood AL. Comment on "First principles electrochemistry: electrons and protons reacting as independent ions". The Journal of Chemical Physics 2005;122(8):87103; discussion 87104.

Ahmed SA, Snow-Bailey K, Highsmith WE, Sun W, Fenwick RG, Mao R. Nine novel germline gene variants in the RET proto-oncogene identified in twelve unrelated cases. Journal of Molecular Diagnostics 2005;7(2):283–8.

Konnick EQ, Williams SM, Ashwood ER, Hillyard DR. Evaluation of the COBAS Hepatitis C Virus (HCV) TaqMan analyte-specific reagent assay and comparison to the COBAS Amplicor HCV Monitor V2.0 and Versant HCV bDNA 3.0 assays. Journal of Clinical Microbiology 2005;43(5):2133–40.

Pounder JI, Williams S, Hansen D, Healy M, Reece K, Woods GL. Repetitive-sequence-PCR-based DNA fingerprinting using the Diversilab system for identification of commonly encountered dermatophytes. Journal of Clinical Microbiology 2005;43(5):2141–7.

Stevenson J, Hymas W, Hillyard D. Effect of sequence polymorphisms on performance of two real-time PCR assays for detection of herpes simplex virus. Journal of Clinical Microbiology 2005;43(5):2391–8.

Aldous WK, Pounder JI, Cloud JL, Woods GL. Comparison of six methods of extracting Mycobacterium tuberculosis DNA from processed sputum for testing by quantitative real-time PCR. Journal of Clinical Microbiology 2005;43(5):2471–3.

Petti CA, Woods CW, Reller LB. Streptococcus pneumoniae antigen test using positive blood culture bottles as an alternative method to diagnose pneumococcal bacteremia. Journal of Clinical Microbiology 2005;43(5):2510–2.

Gouw LG, Reading NS, Jenson SD, Lim MS, Elenitoba-Johnson KS. Expression of the Rho-family GTPase gene RHOF in lymphocyte subsets and malignant lymphomas. British Journal of Haematology 2005;129(4):531–3.

Graham R, Liew M, Meadows C, Lyon E, Wittwer CT. Distinguishing different DNA heterozygotes by high-resolution melting. Clinical Chemistry 2005;51(7):1295–8.

Garavelli L, Cerruti-Mainardi P, Virdis R, Pedori S, Pastore G, Godi M, Provera S, Rauch A, Zweier C, Zollino M, Banchini G, Longo N, Mowat D, Neri G,. Genitourinary anomalies in Mowat-Wilson syndrome with deletion/mutation in the zinc finger homeo box 1B gene (ZFHX1B). Report of three Italian cases with hypospadias and review. Hormone Research 2005;63(4):187–92.

Tripp SR, Willmore-Payne C, Layfield LJ. Relationship between EGFR overexpression and gene amplification status in central nervous system gliomas. Analytical and Quantitative Cytology and Histology 2005;27(2):71–8.

Corpuz GP, Jacobsen RB, Jimenez EC, Watkins M, Walker C, Colledge C, Garrett JE, McDougal O, Li W, Gray WR, Hillyard DR, Rivier J, McIntosh JM, Cruz L. Definition of the M-conotoxin superfamily: characterization of novel peptides from molluscivorous Conus venoms. Biochemistry 2005;44(22):8176–86.

Cairo MS, Raetz E, Lim MS, Davenport V, Perkins SL. Childhood and adolescent non-Hodgkin lymphoma: new insights in biology and critical challenges for the future. Pediatric Blood and Cancer 2005;45(6):753–69.

Rittichier KR, Bryan PA, Bassett KE, Taggart EW, Enriquez FR, Hillyard DR, Byington CL. Diagnosis and outcomes of enterovirus infections in young infants. Pediatric Infections Disease Journal 2005;24(6):546–50.

Pont-Kingdon G, Lyon E. Direct molecular haplotyping by melting curve analysis of hybridization probes: beta 2-adrenergic receptor haplotypes as an example. Nucleic Acids Research 2005;33(10):e89.

Mao R, McDonald J, Cantwell M, Tang W, Ward K. The implication of de novo 21-hydroxylase mutation in clinical and prenatal molecular diagnoses. Genetic Testing 2005;9(2):121–5.

Willmore-Payne C, Holden JA, Tripp S, Layfield LJ. Human malignant melanoma: detection of BRAF- and c-kit-activating mutations by high-resolution amplicon melting analysis. Human Pathology 2005;36(5):486–93.

Hymas W, Stevenson J, Taggart EW, Hillyard D. Use of lyophilized standards for the calibration of a newly developed real time PCR assay for human herpes type six (HHV6) variants A and B. Journal of Virological Methods 2005;128(1–2):143–50.

Litwin CM, Johnson JM. Identification, cloning, and expression of the CAMP-like factor autotransporter gene (cfa) of Bartonella henselae. Infection and Immunity 2005;73(7):4205–13.

Yue B, Lee ED, Rockwood AL, Lee ML. Electron ionization in superimposed magnetic and radio frequency quadrupolar electric fields. Analytical Chemistry 2005;77(13):4160–6.

Yue B, Lee ED, Rockwood AL, Lee ML. Superimposition of a magnetic field around an ion guide for electron ionization time-of-flight mass spectrometry. Analytical Chemistry 2005;77(13):4167–75.

Cloud JL, Carroll KC, Cohen S, Anderson CM, Woods GL. Interpretive criteria for use of AccuProbe for identification of Mycobacterium avium complex directly from 7H9 broth cultures. Journal of Clinical Microbiology 2005;43(7):3474–8.

Layfield LJ, Qureshi MN. HPV DNA testing in the triage of atypical squamous cells of undetermined significance (ASCUS): cost comparison of two methods. Diagnostic Cytopathology 2005;33(2):138–43.

Millson A, Pont-Kingdon G, Page S, Lyon E. Direct molecular haplotyping of the IVS-8 poly(TG) and polyT repeat tracts in the cystic fibrosis gene by melting curve analysis of hybridization probes. Clinical Chemistry 2005;51(9):1619–23.

Owen WE, Roberts WL. Performance characteristics of an HPLC assay for urinary albumin. American Journal of Clinical Pathology 2005;124(2):219–25.

Terry AH, Sandrock T, Meikle AW. Measurement of 25-hydroxyvitamin D by the Nichols ADVANTAGE, DiaSorin LIAISON, DiaSorin RIA, and liquid chromatography-tandem mass spectrometry. Clinical Chemistry 2005;51(8):1565–6.

Caravati EM, Juenke JM, Crouch BI, Anderson KT. Quetiapine cross-reactivity with plasma tricyclic antidepressant immunoassays. Annals of Pharmacotherapy 2005;39(9):1446–9.

Proctor KA, Rowe LR, Bentz JS. Cytologic features of nipple aspirate fluid using an automated non-invasive collection device: a prospective observational study. BMC Womens Health 2005;5:10.

Cloud J L, Hoggan K, Belousov E, Cohen S, Brown-Elliott BA, Mann L, Wilson R, Aldous R, Wallace RJ, Woods GL. Use of the MGB Eclipse system and SmartCycler PCR for differentiation of Mycobacterium chelonae and M. abscessus. Journal of Clinical Microbiology 2005;43(8):4205–7.

Durtschi JD, Erali M, Bromley LK, Herrmann MG, Petti CA, Smith RE, Voelkerding KV. Increased sensitivity of bacterial detection in cerebrospinal fluid by fluorescent staining on low-fluorescence membrane filters. Journal of Medical Microbiology 2005;54(Pt 9):843–50.

Jackson BR, Thomas A, Carroll KC, Adler FR, Samore MH. Use of strain typing data to estimate bacterial transmission rates in healthcare settings. Infection Control and Hosptial Epidemiology 2005;26(7):638–45.

Lewis TB, Robison JE, Bastien R, Milash B, Boucher K, Samlowski WE, Leachman SA, Dirk Noyes R, Wittwer CT, Perreard L, Bernard PS. Molecular classification of melanoma using real-time quantitative reverse transcriptase-polymerase chain reaction. Cancer 2005;104(8):1678–86.

Jackson BR, Roberts WL. Brief report: Free prostate-specific antigen test utilization. Consistency with guidelines. Journal of General Internal Medicine 2005;20(9):859–61.

Flanders MM, Crist RA, Roberts WL, Rodgers GM. Pediatric reference intervals for seven common coagulation assays. Clinical Chemistry 2005;51(9):1738–42.

Heerema NA, Bernheim A, Lim MS, Look AT, Pasqualucci L, Raetz E, Sanger WG, Cairo MS. State of the Art and Future Needs in Cytogenetic/Molecular Genetics/Arrays in childhood lymphoma: summary report of workshop at the First International Symposium on childhood and adolescent non-Hodgkin lymphoma, April 9, 2003, New York City, NY. Pediatric Blood and Cancer 2005;45(5):616–22.

Gunawardena K, Campbell LD, Meikle AW. Antiandrogen-like actions of an antioxidant on survivin, Bcl-2 and PSA in human prostate cancer cells. Cancer Detection and Prevention 2005;29(4):389–95.

Teitell MA, Lones MA, Perkins SL, Sanger WG, Cairo MS, Said JW. TCL1 expression and Epstein-Barr virus status in pediatric Burkitt lymphoma. American Journal of Clinical Pathology 2005;124(4):569–75.

Miles RR, Crockett DK, Lim MS, Elenitoba-Johnson KS. Analysis of BCL6-interacting Proteins by Tandem Mass Spectrometry. Molecular and Cellular Proteomics 2005;4(12):1898–909.

Crockett DK, Lin Z, Vaughn CP, Lim MS, Elenitoba-Johnson KS. Identification of proteins from formalin-fixed paraffin-embedded cells by LC-MS/MS. Laboratory Investigation 2005;85(11):1405–15.

Coles R, Clements TT, Nelson GJ, McMillin GA, Urry FM. Simultaneous analysis of the Delta9-THC metabolites 11-nor-9-carboxy-Delta9-THC and 11-hydroxy-Delta9-THC in meconium by GC-MS. Journal of Analytical Toxicology 2005;29(6):522–7.

McMillin GA, Juenke J, Dasgupta A. Effect of ultrafiltrate volume on determination of free phenytoin concentration. Therapeutic Drug Monitoring 2005;27(5):630–3.

Palais RA, Liew MA, Wittwer CT. Quantitative heteroduplex analysis for single nucleotide polymorphism genotyping. Analytical Biochemistry 2005;346(1):167–75.

Zhou L, Wang L, Palais R, Pryor R, Wittwer CT. High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution. Clinical Chemistry 2005;51(10):1770–7.

Chou LS, Lyon E, Wittwer CT. A comparison of high-resolution melting analysis with denaturing high-performance liquid chromatography for mutation scanning: cystic fibrosis transmembrane conductance regulator gene as a model. American Journal of Clinical Pathology 2005;124(3):330–8.

Liu A, Pasquali M. Acidified acetonitrile and methanol extractions for quantitative analysis of acylcarnitines in plasma by stable isotope dilution tandem mass spectrometry. Journal of Chromatography B- Analytical Technologies in the Biomedical and Life Sciences 2005;827(2):193–8.

Chen Z, Hong B, DeHart S, Heikens M, Tsai S. The Human Penumbra Gene is Mapped to a Region on Chromosome 7 Frequently Deleted in Myeloid Malignancies. Cancer Genetics and Cytogenetics 2005;162(2):95–8.

Chen B, O' Connell CD, Boone DJ, Amos JA, Beck JC, Chan MM, Farkas DH, Lyon E et al. Developing a sustainable process to provide quality control materials for genetic testing. Genetics in Medicine 2005;7(8):534–49.

Park HS, Jin DK, Shin SM, Jang MK, Longo N, Park JW, Bae DS, Bae YS. Impaired generation of reactive oxygen species in leprechaunism through downregulation of Nox4. Diabetes 2005;54(11):3175–81.

Bentz JS. Liquid-based cytology for cervical cancer screening. Expert Rev Molecular Diagnostics 2005;5(6):857–71.

Chen Z, Issa B, Huang S, Aston E, Xu J, Yu M, Brothman AR, Glenn M. A practical approach to the detection of prognostically significant genomic aberrations in multiple myeloma. Journal of Molecular Diagnostics 2005;7(5):560–5.

Fillmore GC, Wang Q, Carey MJ, Kim CH, Elenitoba-Johnson KS, Lim MS. Expression of Akt (protein kinase B) and its isoforms in malignant lymphomas. Leukemia and Lymphoma 2005;46(12):1765–73.

Rawlins ML, Gerstner C, Hill HR, Litwin CM. Evaluation of a Western blot method for the detection of Yersinia Antibodies: evidence of serological cross-reactivity between Yersinia outer membrane proteins and Borrelia burgdorferi. Clinical and Diagnostic Laboratory Immunology 2005;12(11):1269–74.

Willmore C, Holden JA, Layfield LJ. Correlation of HER2 gene amplification with immunohistochemistry in breast cancer as determined by a novel monoplex polymerase chain reaction assay. Applied Immunohistochemistry and Molecular Morphology 2005;13(4):333–41.

Prchal, Josef. Red cell enzymes. Hematology 2005;19–23.

Chou LS, Meadows C, Wittwer CT, Lyon E. Unlabeled oligonucleotide probes modified with locked nucleic acids for improved mismatch discrimination in genotyping by melting analysis. Biotechniques 2005;39(5):644, 646, 648 passim.

Laman CA, Silverstein SB, Rodgers GM. Parenteral iron therapy: a single institution's experience over a 5-year period. Journal of the National Compr Cancer Network 2005;3(6):791–5.

Wallace RJ Jr, Brown-Elliott BA, Brown J, Steigerwalt AG, Hall L, Woods G, Cloud J, Mann L, Wilson R, Crist C, Jost KC Jr, Byrer DE, Tang J, Cooper J,. Polyphasic characterization reveals that the human pathogen Mycobacterium peregrinum type II belongs to the bovine pathogen species Mycobacterium senegalense. Journal of Clinical Microbiology 2005;43(12):5925–35.

Pickering JW, Sant HW, Bowles CA, Roberts WL, Woods GL. Evaluation of a (1->3)-beta-D-glucan assay for diagnosis of invasive fungal infections. Journal of Clinical Microbiology 2005;43(12):5957–62.

Petti CA, Polage CR, Schreckenberger P. The role of 16S rRNA gene sequencing in identification of microorganisms. Journal of Clinical Microbiology 2005;43(12):6123–5.

Neal H, Cloud JL, Pounder JI, Page SR, Woods GL. Sequence variant for internal transcribed spacer region of Mycobacterium abscessus. Journal of Clinical Microbiology 2005;43(12):6214.

Perkins SL, Pickering D, Lowe EJ, Zwick D, Abromowitch M, Davenport G, Cairo MS, Sanger WG. Childhood anaplastic large cell lymphoma has a high incidence of ALK gene rearrangement as determined by immunohistochemical staining and fluorescent in situ hybridisation: a genetic and pathological correlation. British Journal of Haematology 2005;131(5):624–7.

Yang DT, Greenwood JH, Hartung L, Hill S, Perkins SL, Bahler DW. Flow cytometric analysis of different CD14 epitopes can help identify immature monocytic populations. American Journal of Clinical Pathology 2005;124(6):930–6.

Lundell R, Hartung L, Hill S, Perkins SL, Bahler DW. T-cell large granular lymphocyte leukemias have multiple phenotypic abnormalities involving pan-T-cell antigens and receptors for MHC molecules. American Journal of Clinical Pathology 2005;124(6):937–46.

Crockett DK, Seiler CE, Elenitoba-Johnson KSJ, Lim MS. Annotated proteome of a human T-cell lymphoma. Journal of Biomolecular Tech 2005;16(4):341–6.

Rockwood AL, Crockett DK, Oliphant JR, Elenitoba-Johnson KSJ. Sequence alignment by cross-correlation. Journal of Biomolecular Techniques 2005;16(4):453–8.