COVID-19

Resources for Clinicians and Your Patients


Page updated Sept 21, 2021Linkedin Email

 

SARS-CoV-2, the virus that causes COVID-19, continues to spread in the United States and many other countries, straining the capacity of healthcare systems in some states to treat patients with the infection. Older adults remain most at risk for hospitalization due to severe COVID-19 disease, but younger individuals are more responsible for the spread of infection than they were in the winter and spring of 2020.

Viral detection testing is recommended for COVID-19 diagnosis. Testing decisions should be based on local epidemiology, clinical signs and symptoms, and the course of illness. Antibody testing can be used to evaluate exposure to SARS-CoV-2, but is not recommended for diagnosis of acute illness.

 

Quick Answers for Clinicians

Which tests can be used to detect vaccine-induced immune thrombotic thrombocytopenia?

New April 28, 2021

Patients with vaccine-induced immune thrombotic thrombocytopenia (VITT) have been described as having platelet-activating antibodies detectable with PF4-heparin ELISA assays, and possibly with functional assays such as the serotonin release assay. Only a limited number of patients have been described to date, and testing algorithms remain in development. For more information, please refer to the Vaccine-Induced Immune Thrombotic Thrombocytopenia Testing section in the ARUP Consult COVID-19 topic.

When is it appropriate to perform combined testing (cotesting) for SARS-CoV-2, respiratory syncytial virus, and influenza?

Updated November 23, 2020

Laboratory testing is the only way to distinguish between infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other infections with similar symptoms. Furthermore, laboratory testing is the only way to determine cases of viral coinfection.1 Patients may be infected with multiple viruses at the same time, so diagnosis of infection with one respiratory virus does not exclude the possibility of infection with another virus.2,3 The National Institutes of Health (NIH) recommends cotesting to determine proper medical management if multiple viruses (eg, SARS-CoV-2, respiratory syncytial virus (RSV), and influenza A/B) are circulating, such as during flu season.1,3

In which scenarios is viral detection testing (eg, nucleic acid amplification [NAA], polymerase chain reaction [PCR], antigen testing) recommended?

Updated September 14, 2020

Viral testing for SARS-CoV-2 infection may be used for diagnostic purposes (eg, when testing individuals with symptoms consistent with COVID-19), screening purposes (eg, when testing asymptomatic individuals with known or suspected recent exposure to SARS-CoV-2), and surveillance purposes (eg, when testing asymptomatic individuals to detect transmission hot spots or characterize disease trends). Nucleic acid amplification (NAA) testing is the gold standard for detection of SARS-CoV-2 virus.4,5

Do existing respiratory virus panels detect SARS-CoV-2?

Updated November 23, 2020

Yes. Some multipathogen molecular assays can detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).2 Clinicians are advised to confirm which respiratory viruses are detected by an assay before ordering. The U.S. Food and Drug Administration (FDA) maintains a list of COVID-19 assays with Emergency Use Authorization (EUA) that includes respiratory virus panels.

What is the latest guidance from the CDC regarding who should be tested for COVID-19?

Updated June 1, 2020

The latest CDC guidance for SARS-CoV-2 testing can be found in the CDC’s Overview of Testing for SARS-CoV-2 (COVID-19).4 According to the CDC, viral detection testing (eg, NAA, antigen testing) is recommended for individuals with signs or symptoms consistent with COVID-19.4 Testing may also be incorporated as a part of transmission reduction strategies. Diagnostic testing for asymptomatic individuals with known or suspected recent exposure to SARS-CoV-2 may also be advised.4

Local and regional health authorities may provide additional guidance for prioritizing patients for COVID-19 testing. Clinicians are also encouraged to consider testing for other causes of respiratory illness, including influenza.

When is serology testing recommended?

Updated May 23, 2020

Antibody testing is not currently recommended to diagnose infection or to infer an individual’s immunity to the virus. It may aid in determining the rate of exposure in a given population. Antibody testing can also help identify individuals who have been exposed to SARS-CoV-2 in order to qualify potential convalescent plasma donors. COVID-19 convalescent plasma is currently being studied as a possible treatment for individuals who are critically ill with COVID-19 and as a prophylactic means of protecting individuals at high risk of exposure.6

How does COVID-19 affect children compared with adults?

Updated September 14, 2020

Children of all ages are at risk for COVID-19 infection, but there are relatively fewer cases of COVID-19 among children compared with adults. Children appear to present with more mild signs and symptoms than adults.7 Although severe disease is uncommon, children are still at risk of developing severe illness and complications from COVID-19. Early case studies and reports suggest that infants may be at a higher risk for severe illness from COVID-19 when compared with older children.7

It is unclear whether children are as susceptible to SARS-CoV-2 infection compared with adults. Children can transmit the virus, but data are limited as to whether they can transmit the virus as effectively as adults. Recent studies have suggested that young children (those younger than 5 years) have higher viral loads in their nasopharynxes as compared with adults8 and that children can spread the virus effectively in households and camp settings.9

Due to early community mitigation efforts and school closures, transmission of SARS-CoV-2 to and among children may have been reduced in the U.S. during the pandemic in the spring and early summer of 2020.

What is multisystem inflammatory syndrome in children (MIS-C), and how is it related to COVID-19?

Updated September 14, 2020

The CDC is investigating reports of multisystem inflammatory syndrome in children (MIS-C), a serious condition marked by inflammation that may be related to resolved COVID-19 infection.

At this time, there is limited information available about risk factors, pathogenesis, and clinical course. The CDC has issued a health advisory instructing clinicians to watch for signs and symptoms, which may include a persistent fever, elevated inflammatory markers, and multiorgan (eg, cardiac, gastrointestinal, renal) involvement.7 For more information, refer to the CDC’s case definition for MIS-C.10

Which specimens are acceptable for COVID-19 molecular viral detection testing?

Updated April 15, 2021

Nasopharyngeal (NP) specimens are the gold standard for COVID-19 viral detection. Some laboratories may accept alternative specimen types such as saliva, oropharyngeal (OP) swabs, midturbinate swabs, or anterior nares swabs.5,11 The CDC Specimen Collection Guidelines contain detailed information about EUA-approved specimen types.12 Clinicians are advised to check with their performing laboratory for specific specimen requirements.

Recent studies, including one performed by researchers at ARUP and University of Utah Health,13 found that self-collected saliva and NP swabs collected by healthcare providers are equally effective for SARS-CoV-2 detection. Both saliva and NP swabs are superior to anterior nasal swabs. The study, published in the Journal of Clinical Microbiology, represents one of the largest COVID specimen-type comparisons to date.13 However, saliva specimens are not suitable for all tests that detect SARS-CoV-2.

Detection rates in specimen types vary from patient to patient and may change over the course of the illness.14 For example, because of potentially discordant shedding of virus in the upper versus the lower respiratory tract, patients with pneumonia may have negative nasal or OP samples but positive lower airway samples.15,16

Point-of-care antibody tests use fingerstick whole blood samples. Serum or plasma specimens are generally required for antibody tests that are analyzed in a laboratory.6

Which specimens are acceptable for COVID-19 serology testing?

New April 15, 2021

Serum and plasma are the standard specimen types for COVID-19 serology testing. Most commercial assays, including those utilized at ARUP Laboratories, are only validated in these specimen types.17 Alternate specimen types, such as cerebrospinal fluid (CSF) and cord blood, are inappropriate for use in assays for which they have not been validated. Clinicians are advised to check with their performing laboratory for specific specimen requirements.

Recently, the CDC approved the use of capillary fingerstick specimen collection for some point-of-care assays. The CDC Specimen Collection Guidelines12 contain more detailed information about EUA-approved specimen types.

Which collection media is preferred for COVID-19 respiratory samples?
Which collection media is preferred for saliva specimens?

Updated September 14, 2020

Swab specimens should be collected with NP ultrafine or equivalent swabs. Dacron, polyester-tipped, or any other flocked swabs are acceptable alternatives. Calcium alginate swabs or swabs with wooden shafts are NOT acceptable due to test interference. Viral transport media and universal transport media (VTM/UTM) are the preferred collection systems for swabs. Media types that are equivalent to VTM/UTM are also acceptable.

Saliva specimens are self-collected in ARUP’s saliva collection tubes. This method reduces healthcare providers’ risk of exposure during the collection process. The patient should not eat or drink for 30 minutes prior to providing a saliva sample. Saliva should not surpass the fill line on the tube. Saliva specimens must be collected in the presence of a healthcare provider.

For more information, refer to ARUP’s COVID-19 Specimen Collection Guide.12 For alternative transport media, refer to the FDA’s guidance on specimen collection for SARS-CoV-2 molecular diagnostic testing.11

What laboratory testing is useful to evaluate long-haul COVID-19 symptoms and/or post-COVID-19 conditions?

New September 21, 2021

COVID-19 diagnostic (nucleic acid amplification) or serologic testing is useful for determining current or past SARS-CoV-2 infection status but is not useful in the evaluation of post-COVID-19 conditions. The CDC provides interim guidance for laboratory testing to evaluate patients with post-COVID-19 conditions.18 In short, the CDC recommends that laboratory testing should be informed by a detailed evaluation of patient history, physical examination, and clinical findings. Basic laboratory tests (e.g., blood count, liver function, etc.) may be informative.

 
Decision Support Tools
Clinical Resources

Testing Guidance

Specimen Collection

Refer to the COVID-19 Specimen Collection Guide for detailed information on specimen collection and transport media. In addition, healthcare provider fact sheets for the EUA SARS-CoV-2 NAA test offered by ARUP are available on the Test Information for Hospitals and Labs page, and specimens should be collected following the CDC’s Interim Guidelines for Collecting, Handling, and Testing Clinical Specimens for COVID-19. Healthcare providers also may find the following videos useful to help demonstrate specimen collection for SARS-CoV-2 testing.

COVID-19 Journal Article Publications

  • LitCOVID
    • A curated literature hub for tracking up-to-date scientific information, hosted by the NIH
References
  1. National Institutes of Health. Influenza and COVID-19. [Updated: Oct 22, 2020; Accessed: Sep 20, 2021]
  2. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Coronavirus Disease 2019 (COVID-19). Clinical care guidance. [Updated: Feb 12, 2021; Accessed: Sep 20, 2021]
  3. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Coronavirus Disease 2019 (COVID-19). FAQs. [Updated: Mar 4, 2021; Accessed: Sep 20, 2021]
  4. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Coronavirus Disease 2019 (COVID-19). Testing overview. [Updated: Aug 2, 2021; Accessed: Sep 20, 2021]
  5. Hanson KE, Caliendo AM, Arias CA, et al. Infectious Diseases Society of America guidelines on the diagnosis of COVID-19. [Published: Dec 23, 2020; Accessed: Sep 20, 2021]
  6. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Coronavirus Disease 2019 (COVID-19). Antibody testing interim guidelines. [Updated: Mar 17, 2021; Accessed: Sep 20, 2021]
  7. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Coronavirus Disease 2019 (COVID-19). Children. [Updated: Dec 30, 2020; Accessed: Sep 20, 2021]
  8. Heald-Sargent T, Muller J, Zheng X, et al. Age-related differences in nasopharyngeal severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) levels in patients with mild to moderate coronavirus disease 2019 (COVID-19). JAMA Pediatr. 2020;174(9):902-903.
  9. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. SARS-CoV-2 transmission and infection among attendees of an overnight camp – Georgia, June 2020. MMWR. 69(31);1023-1025.
  10. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Multisystem Inflammatory Syndrome (MIS).. [Last reviewed: May 20, 2021; Accessed: Sep 20, 2021]
  11. U.S. Department of Health and Human Services, Food and Drug Administration. FAQs on testing for SARS-CoV-2. [Last reviewed: Mar 25, 2021; Accessed: Sep 20, 2021]
  12. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Specimen collection. [Updated: Feb 26, 2021; Accessed: Sep 20, 2021]
  13. Hanson KE, Barker AP, Hillyard DR, et al. Self-collected anterior nasal and saliva specimens versus healthcare worked-collected nasopharyngeal swabs for the molecular detection of SARS-CoV-2. J Clin Microbiol. 2020;58(11):e01824-20.
  14. Wang W, Xu Y, Gao R, et al. Detection of SARS-CoV-2 in different types of clinical specimens. JAMA. 2020;323(18):1843-1844.
  15. Patel R, Babady E, Theel ES, et al. Report from the American Society for Microbiology COVID-19 International Summit, 23 March 2020: value of diagnostic testing for SARS-CoV-2/COVID-19. mBio. 2020;11(2):e00722-20.
  16. National Institutes of Health. Testing for SARS-CoV-2 Infection. [Updated: April 21, 2021; Accessed: Sep 20, 2021]
  17. Zhang YV, Wiencek J, Meng QH, et al. AACC practical recommendations for implementing and interpreting SARS-CoV-2 EUA and LDT serologic testing in clinical laboratories. Clin Chem. 2021 [Published online ahead of print Mar 2021].
  18. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Evaluating and caring for patients with post-COVID conditions: interim guidance. [Updated: Jun 14, 2021; Accessed: Sep 20, 2021]