Immunohistochemistry Stains

The ARUP Immunohistochemistry Laboratory performs more than 175 stains on formalin-fixed, paraffin-embedded tissues/cellblocks. These stains are used to detect the presence, abundance, and localization of specific proteins to aid in determining the direction of differentiation in neoplasms with similar morphology as well as to provide prognostic or therapeutic information, among other applications.

This lab is pathologist oriented, providing a stain and return service only, without interpretation, to our client pathologists to assist in their diagnostic studies. Most stains are returned the next day. The available stains are listed under the Stain and Return Immunohistochemistry tab. If interpretation is needed, please order a consultation.

To order a surgical pathology consultation, please use test code 2013263 Consultation, Surgical Pathology or 2013258 Consultation, Hematopathology. For more information, call (801)581-2507. Please include pertinent clinical history and surgical pathology report.

The Immunohistochemistry Laboratory has a menu of immunohistochemistry tests for which we will provide interpretation by one of our ARUP faculty pathologists. A list of these tests can be found under the Immunohistochemistry Tests tab. The ARUP Immunohistochemistry Laboratory is an NSABP-approved laboratory for the testing of breast markers.
All A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Test # Test Name Test Keywords Test Description
Consultation, Muscle/Nerve Pathology Special Study
2003430 Adenovirus by Immunohistochemistry Adenovirus
  • Specific to all subtypes of adenovirus
2003427 Adrenocorticotropic Hormone (ACTH) by Immunohistochemistry Acromegaly, Cushing Syndrome, Hypopituitarism, Tumor Markers, Endocrine/Adrenal Cancer, Lung Cancer, Pituitary Cancer, thymoma, medullary thyroid carcinoma Adrenocorticotropic hormone:
  • Subclassifies pituitary adenomas
2003433 Pan Cytokeratin (AE1,3) by Immunohistochemistry Glucagonoma, Somatostatinoma, VIPoma, Gastrinoma, Pancreatic Neuroendocrine Tumors (NET), Mesothelioma, Testicular Cancer, Tumor Markers, Synovial Sarcoma, Epithelial Sarcoma, Carcinoma, Adenocarcinoma, Tumor Markers, AE1,3
  • Cytokeratin antibody cocktail for acidic and basic cytokeratins
2003418 Alpha-1-Antichymotrypsin (A1ACT) by Immunohistochemistry Pancreatic Acinar Carcinoma, Alpha-1 Antitrypsin Deficiency, Tumor Markers Alpha 1 ACT:
  • Aids in identifying hepatomas and some germ cell neoplasms
  • Histiocyte marker for normal/neoplastic tissue
2003424 Alpha-1-Antitrypsin (AAT) by Immunohistochemistry Alpha-1-Antitrypsin Deficiency (AAT), Hemochromatosis Alpha 1 AT:
  • Expressed by cells of histolytic origin
  • Aids in identifying germ cell and histolytic neoplasms, as well as embryonal and some lung carcinomas
2003436 Alpha-1-Fetoprotein (AFP) by Immunohistochemistry Testicular Germ Cell tumor, Ovarian Germ Cell Tumor, Brain Tumors, Tumor Markers
  • Expressed by neoplastic liver and gonad tissue
  • Aids in identifying bladder carcinomas, yolk sac tumors, some germ cell tumors and a high proportion of hepatocellular carcinoma
2003419 Alpha-Synuclein by Immunohistochemistry aSynuclien (SNCA):
  • Demonstrates Lewy bodies in brain cells associated with Parkinson's and Alzheimer's.
2003439 Anaplastic Lymphoma Kinase 1 (ALK-1) by Immunohistochemistry Sarcoma, T/NK-Cell Lymphomas, B-Cell Lymphomas, Tumor Markers, Non-small cell carcinoma, lung, malignant rhabdoid tumor Anaplastic lymphoma kinase 1:
  • Reacts with the NPM-ALK fusion protein expressed by t(2;5) positive anaplastic large cell lymphomas
2011890 Arginase 1 by Immunohistochemistry
  • Aides in the distinction of HCC from other hepatocellular and non-hepatocellular mass lesions, as well as in cases of metastatic carcinoma and other benign and malignant nonhepatocellular mimics
2014499 ATRX by Immunohistochemistry Prostate Cancer, prostate epithelium, breast, ductal epithelium, gastric mucosa, glial cells
  • Expression of ATRX is implicated in cancer pathogenesis and is useful in the diagnosis of astrocytic gliomas. Its specificity and prevalence in lower-grade gliomas with an IDH-mutation argue for thorough characterization of associated signaling networks to facilitate therapeutic development.
  • Mutation or loss of alpha-thalassemia/mental retardation syndrome X-linked (ATRX) expression has been described in anaplastic gliomas. ATRX loss is a hallmark of astrocytic tumors and defines a subgroup of astrocytic tumors with a favorable prognosis.
2003442 B Cell Specific Octamer Binding Protein-1 (BOB-1) by Immunohistochemistry B-Cell Lymphomas, Tumor Markers B-cell Oct-binding protein 1; OBF-1:
  • Expressed in spleen and peripheral blood leukocytes, Reed-Sternberg cells, B-cells, and germinal centers
  • Aids in differentiating Hodgkin lymphomas and B-cell lymphomas
2004513 BCL-2 by Immunohistochemistry Follicular lymphoma, Diffuse large B-cell lymphoma, B-Cell Lymphomas B-cell lymphoma-2:
  • Proto-oncogene
  • Over expression increases life span in B-cells
  • Aids in identifying colorectal adenomas, carcinomas
  • Distinguishes follicular lymphoma from reactive follicles
2003457 BCL-6 by Immunohistochemistry B-Cell Lymphomas, Tumor Markers
  • Transcription factor important in germinal center formation
  • Aids in identifying large cell lymphomas, Burkitt lymphoma, and Hodgkin lymphoma (nodular, lymphocyte predominant)
2003463 Anti-Human Epithelial Antigen, Ber-EP4 by Immunohistochemistry Mesothelioma, Lung Cancer, mammary Paget disease, lung adenocarcinomas, trichoepitheliomas, dermatofibromas, basal cell carcinoma, Lung cancer, mesothelioma
  • Epithelial cell membrane glycoprotein
  • Differentiates mesothelial from epithelial cells
  • Aids in identifying mammary Paget disease, lung adenocarcinomas, trichoepitheliomas, dermatofibromas, basal cell carcinomas, and other carcinomas
2003454 Beta-Catenin-1 by Immunohistochemistry Solid-pseudopapillary Neoplasm of Pancreas, Desmoid-type Fibromatosis, Ovarian Cancer, Tumor Markers, Stain and return service only type
  • Binds to cytoplasmic region of e-cadherin molecule
  • Plays a role in cell adhesion, signal transmission, and actin cytoskeleton anchoring
  • Aids in identifying skin, liver, ovary, brain, prostate, and some breast cancers, as well as endometrial, ovarian, and colon carcinomas
2003466 BF-1 by Immunohistochemistry T/NK-Cell Lymphomas, Tumor Markers Beta framework 1; BF-1:
  • Recognizes T-cell receptor (TCR) beta subunit
  • Aids in characterizing alpha-beta T-cell receptors from T-cell clones or polyclonal populations of T-cells
  • Aids in diagnosing T-cell lineage neoplasms
2003472 Breast 2 (GCDFP-15) by Immunohistochemistry Tumor Markers, Mammary carcinoma, Extramammary Pagets disease
  • Gross cystic disease fluid protein-15
  • Produced by cells with apocrine function
  • Differentiation marker for mammary carcinomas
  • Extramammary Paget disease
2003445 Breast Carcinoma b72.3 by Immunohistochemistry Mesothelioma, Lung Cancer, Tumor Markers, Adenocarcinoma, Breast carcinoma Tumor-associated glycoprotein (TAG.72):
  • Recognizes tumor-associated oncofetal antigen
  • Aids in identifying adenocarcinomas and breast carcinomas
2008652 c-MET by Immunohistochemistry
  • Tumors derived from c-Met expressing epithelia are usually positive; these include colorectal carcinomas, gastric adenocarcinomas, and non-small cell lung carcinomas
  • In gastric cancer and non-small cell lung carcinoma, it has been determined that c-MET drives the cancer
  • It has also been found that c-MET is a resistance pathway in lung cancer for EGFR inhibitors
  • This antibody may be used to aid in the identification of normal and neoplastic c-MET expressing cells. The pattern of reactivity is cytoplasmic/membranous
2008317 C-MYC by Immunohistochemistry
  • c-MYC expression has been described in a variety of cancers including breast cancer, prostate cancer, lymphoma, lung and colon cancers
  • The c-MYC antibody may be used to characterize lymphomas
  • The pattern of reactivity is nuclear
2003475 C4D by Immunohistochemistry
  • Deposits in peritubular capillary might distinguish between Acute Humoral Rejection (AHR) and Acute Cellular Rejection (ACR).
  • AHR: deposits prominently and diffusely in the peritubular capillaries, intense staining has been seen in a widespread, uniform distribution
2003481 Calcitonin by Immunohistochemistry Medullary Thyroid Cancer, Tumor Markers, Endocrine/Adrenal Cancer, Head and Neck Cancer, Thyroid medullary carcinomas
  • Thyroid parafollicular cells (C-cells)
  • Thyroid medullary carcinomas
2003484 Caldesmon by Immunohistochemistry Gastrointestinal Stromal Tumors, GIST, endometrial, stromal, smooth muscle tumors, Tumor Markers
  • High molecular weight isoform is H-caldesmon
  • Calcium-, calmodulin-, tropomyosin-, and actin-binding protein
  • Regulates smooth muscle contraction
  • Aids in identifying angioleiomyomas, glomus tumors, GIST, some endometrial stromal tumors, and uterine smooth muscle tumors (benign or malignant)
  • Differentiates uterine smooth muscle (usually positive) and endometrial stromal differentiation (usually negative)
2003487 Calponin by Immunohistochemistry
  • Can be used to characterize the differentiation process of mammary myoepithelial cells in the developing mammary glad, investigate the nature of myoepithelial cells and to study the development of human smooth muscle cells.
  • High expression might be used as an additional marker of vascular smooth muscle cells, myopithelial cells in normal and benign human mammary gland, and certain stromal myofibroblasts.
2003490 Calretinin by Immunohistochemistry Mesothelioma, Ovarian Cancer, Tumor Markers
  • Calcium-binding protein
  • Mesothelial cells: 89% sensitive, 90% specific for differentiating mesotheliomas from lung adenocarcinomas
  • Aids in identifying mesothelial hyperplasia and epithelioid mesotheliomas
2003824 Carcinoembryonic Antigen, Monoclonal (CEA M) by Immunohistochemistry Mesothelioma, Ovarian Cancer, Colorectal Cancer, Tumor Markers, Cervical Cancer Carcinoembryonic antigen:
  • Specific marker for colon carcinoma
  • Associated with other cancers: breast, stomach, and lung (see CEA polycolonal)
2003827 Carcinoembryonic Antigen, Polyclonal (CEA P) by Immunohistochemistry Hepatocellular Carcinoma, canallicular marker, Pancreatic Cancer, Ovarian Cancer, Lung Cancer, Colorectal Cancer, Tumor Markers CD56e; biliary glycoprotein 1:
  • Reacts with CEA and CEA-like proteins
  • Normal colon: small intestinal crypts and apical surfaces of epithelial cells, small intestinal goblet cell mucin (not intracytoplasmic)
  • Breast: some epithelial cell membranes expressed
  • Liver: biliary tract, hepatocytes are positive
  • Lung adenocarcinomas or mesotheliomas: 85% sensitive, 96% specific for lung adenocarcinoma, diffuse cytoplasmic staining with membrane enhancement
  • Hepatocellular or non-hepatocellular carcinomas: canalicular pattern has 50-90% sensitivity for hepatocellular carcinoma and greater than 95% specificity
  • Present also with lung adenocarcinoma, medullary thyroid carcinoma, colonic adenocarcinoma, pancreatic adenocarcinoma, and hepatocellular carcinoma
2003523 CD10 (CALLA) by Immunohistochemistry Acute Lymphoblastic Leukemia (ALL), Follicular lymphoma, Burkitt lymphoma, Diffuse large B-cell lymphoma, B-Cell Lymphomas, Tumor Markers
  • Common acute lymphoblastic leukemia antigen (CALLA)
  • Aids in identifying lymphoblastic lymphoma, Burkitt lymphoma, follicular lymphoma, and CML
  • Aids in differential diagnosis of small B-cell lymphomas and subtyping of lymphoblastic leukemias
2003806 CD117 (c-Kit) by Immunohistochemistry Gastrointestinal Stromal Tumors (GIST), Lung Cancer, Breast Cancer, Melanoma, Mast Cell Disease, Germ Cell Tumor of Testes and Ovary, Brain Tumors, Myelodysplastic Syndromes, Acute Myeloid Leukemia, (AML), Myeloproliferative Neoplasms (MPN), Tumor Markers
  • Proto-oncogene
  • Inhibitor of apoptotic cell death
  • Aids in identifying small and large cell lung carcinomas, GIST, AML, CML (in blast crisis), breast carcinomas, glioblastomas and melanomas
2003809 CD123 by Immunohistochemistry
  • A marker of dendric cell precursors: is expressed by plasmactoid monocytes, dendric cells, and plasmacytoid dendritic cells.
2003812 CD138 (Syndecan-1) by Immunohistochemistry Plasma Cell Dyscrasias, B-Cell Lymphomas, Tumor Markers
  • Transmembrane heparin sulphate glycoprotein
  • Expressed by normal lymphoid cells, IgG plasma cells, Pre B-cells and immature B-cells
  • Co-receptor for differentiation growth factors
  • Differentiates squamous cell carcinomas, postgerminal center B-cells, and plasma cells
  • Expressed in plasma cells of CLL, plasmacytoid lymphomas, and myelomas
2003526 CD14 by Immunohistochemistry Acute Myelogenous Leukemia, Chronic Myelomonocytic Leukemia, Tumor Markers
  • Acts as a co-receptor for the detection of bacterial lipolysaccharide (LPS).
  • Expressed on cells of myelo-monocytic llineage, including monocytes, macrophages and Langerhan's cells.
2003529 CD15, Leu M1 by Immunohistochemistry Hodgkin Lymphoma, Acute Myelogenous Leukemia, Mesothelioma
  • Hapten X
  • Present in Reed-Sternberg cells, 90% granulocytes, 30-60% monocytes
  • Absent from lymphocytes
  • Expressed by some non-Hodgkin lymphomas, mycosis fungoides and some leukemias
2003815 CD163 by Immunohistochemistry
  • Is restricted in its expression to the monocytic/macrophage lineage.
  • Is present on all circulating monocytes and most tissue macrophages except those found in mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan’s cells.
2005114 CD19 by Immunohistochemistry Acute Lymphoblastic Leukemia, Tumor Markers, B-Cell Lymphomas CD19:
  • CD19 is to be used in a panel of antibodies to aid in the characterization of B-cell malignancies. The pattern or reactivity is membranous.
  • In normal tissues, the CD19 (clone BT51E) detects protein expressed on the membrane of cells of the B-cell lineage. Staining is seen in the mantle zones and germinal centers of tonsil and tissue infiltrating B-lymphocytes.
  • CD19 is detected in hematological malignancies including: 95% of B-cell lymphomas including small lymphocytic lymphoma, chronic lymphocytic lymphoma, mantle cell lymphoma, follicular lymphoma, Burkitt lymphoma, marginal zone lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, and lymphoblastic lymphoma; 75% of B-cell leukemias including small lymphocytic leukemia, chronic lymphocytic leukemia and Hairy cell leukemia.
2003502 CD1a by Immunohistochemistry Langerhan cell histiocytosis, T-ALL
  • Non-polymorphic MHC class I-related cell surface glycoprotein
  • Expressed by interdigitating reticulum cells, cortical thymocytes, thymomas, langerhans cells and Langerhans histiocytosis cells (histiocytosis X)
  • Aids in identifying some T-cell lymphomas and leukemias
2003505 CD2 by Immunohistochemistry T-cell lymphomas, Mast Cell Disease, Sézary Syndrome, Tumor Markers
  • Mediates adhesion of activated T-cells and thymocytes with antigen-presenting and target cells
  • Expressed by T-lymphocytes and cortical thymocytes
  • Aids in identifying natural killer cells and most malignant cells of T-cell origin
2003532 CD20, L26 by Immunohistochemistry B-Cell Lymphomas, Plasma cell dyscrasias, Tumor Markers
  • Expressed on B-cell precursors and mature B-cells, but lost following differentiation into plasma cells
  • B-lymphocytes: germinal center cells, mantle zone lymphocytes, and interfollicular lymphocytes, but not T-cells, plasma cells, or histiocytes
  • Aids in identifying common acute lymphoblastic leukemia, pre-B acute lymphoblastic leukemia, CLL, prolymphocytic leukemia, hairy cell leukemia, lymphoma cell leukemia, B-cell lymphomas, including Burkitt, Waldenstrom, and immunoblastic B-cell
  • May show some membrane staining of Reed-Sternberg cells in Hodgkin lymphoma
2012844 CD200 by Immunohistochemistry
  • Primarily aids the distinction between CLL/SLL and mantle cell lymphoma where CD200 is usually positive in CLL/SLL and negative in mantle cell lymphoma.
  • CD200 is also positive in other B-cell lymphoproliferative disorders.
2003535 CD21 (Dendritic Cell) by Immunohistochemistry T-Cell Lymphomas, B-Cell Lymphomas, follicular dendritic cells, follicular dendritic sarcoma
  • C3D receptor, CR2, and EBV receptor
  • Expressed by follicular dendritic cells, mature B-cells, some types of epithelial cells, reactive hyperplasia, and plasma cells
  • Low expression on T-cell ALL cells, subset of normal thymocytes and mature T-cells, lymphocytes in the mantle zone, sinus lining cells, and monocytoid B-cells
  • Aids in identifying B-cell CLL, follicular lymphoma, low-grade MALT-type B-cell lymphoma, primary salivary gland and gastric lymphoma, T-cell and histiocyte-rich B-cell lymphoma, angioimmunoblastic T-cell lymphoma, nodular lymphocyte-predominant Hodgkin lymphoma, follicular dendritic sarcoma and some Reed-Sternberg cells not expressing other B- or T-cell-associated markers.
2003541 CD23 by Immunohistochemistry Chronic Lymphocytic Leukemia
  • Expressed by activated B-lymphocytes, activated macrophages, and a portion of follicular dendritic cells
  • Aids in differentiating small lymphocytic lymphoma (+) and mantle cell lymphoma (-)
2003544 CD25 by Immunohistochemistry Mast Cell Disease, Myeloproliferative Neoplasms (MPN), Hairy cell leukemia, Sezary syndrome
  • Mediates helper, suppressor, and cytotoxic functions
  • Expressed on T-cells, HTLV-1-transformed T- and B-cells, EVB-transformed B-cells, myeloid precursors, and oligodendrocytes
  • Aids in study of inflammatory and malignant conditions
2003508 CD3 by Immunohistochemistry T-cell lymphomas, T-cell leukemias, Tumor Markers
  • Binds to the non-glycosylated epsilon chain of CD3 complex
  • Present in T-lymphocytes, thymocytes/early thymocytes
  • Expressed on normal and neoplastic T-cells
2003547 CD30 (Ki-1) by Immunohistochemistry Hodgkin lymphoma, T-cell lymphoma, B-Cell Lymphomas, Tumor Markers, solitary fibrous tumor, sarcoma
  • Ki-1 positive lymphoma
  • Ber-H2
  • Expressed by activated B- and T-lymphocytes, Reed-Sternberg cells
  • Aids in identifying Hodgkin lymphoma, lymphomatoid papulosis, anaplastic large cell lymphoma, some peripheral pleomorphic T-cell lymphomas (both HTLV-1 positive and negative) including those of angioimmunoblastic type and Lennert type and embryonal carcinoma
2003550 CD31 by Immunohistochemistry Vascular neoplasms
  • Platelet-endothelial cell adhesion molecule
  • Expressed by endothelial cells
  • Glycoprotein in platelets
  • Aids in evaluating vascularization in normal and neoplastic tissue and demonstrating endothelial cellderived tumors
2003553 CD33 by Immunohistochemistry Acute Myeloid Leukemia (AML), Tumor Markers
2003556 CD34, QBEnd/10 by Immunohistochemistry B-ALL, T-ALL, Acute myelogenous leukemia, MDS, Tumor Markers
  • Human progenitor cell antigen
  • Present in immature hematopoietic cells and vascular endothelial cells
  • Expressed by some acute myeloid leukemias, undifferentiated leukemias, and acute lymphoblastic leukemias
2003559 CD35 by Immunohistochemistry Sarcoma
  • Mediates neutrophil and monocte phagocytosis of particles coated with C3b and or C4b
  • Shows strong staining pattern on follicular dendritic cells
  • Aids in characterization of histocytic/dendritic cell neoplasms and follicular dendritic cell sarcomas
2003511 CD4 by Immunohistochemistry Sézary Syndrome, T-Cell Lymphomas, Tumor Markers
  • Present on a subset of helper/inducer T-cells, thymocytes, and at a lower level on monocytes
  • Aids in identifying cutaneous T-cell lymphoma, including mycosis fungoides and HTLV-1-associated adult T-cell leukemia and lymphoma
2003565 CD42b by Immunohistochemistry Acute myelogenous leukemia, megakaryocytes
  • Platelet glycoprotein Ib
  • Expressed on platelets and megakaryocytes in bone marrow
  • Aids in phenotyping megakaryoblastic leukemias
  • Absence of CD42b on platelets may indicate Bernard-Soulier syndrome
2003568 CD43, L60 (Leu 22) by Immunohistochemistry T/NK-Cell Lymphomas, B-Cell Lymphomas
  • Expressed on T-lymphocytes, B-lymphocytes, and granulocytes
  • Aids in identifying cells of lymphoid lineage and T-cell lymphomas, and mantle cell lymphomas
2003574 CD45 by Immunohistochemistry Non-Hodgkin lymphomas, Hodgkin Lymphomas, Tumor Markers Leukocyte common antigen:
  • Expressed on the majority of leukocytes
  • Aids in differentiating lymphoid from non-lymphoid neoplasms
2003514 CD5 by Immunohistochemistry T-Cell Lymphomas, B-Cell Lymphomas, Tumor Markers
  • Expressed in T-lymphocytes, thymocytes and subset of B-lymphocytes in lymph nodes
  • Aids in identifying T-cell leukemias, most B-cell chronic lymphocytic leukemias (B-CLL) and mantle cell lymphomas/T- and B-cell lymphomas
2003586 CD52 (CAMPATH-1) by Immunohistochemistry B-cell lymphomas, B-cell leukemias, T-cell leukemias
  • Expressed by lymphocytes, monocytes, eosinophils, thymocytes, and macrophages
  • Expressed by most lymphoid-derived malignancies
  • Variable expression on myeloma cell
2003589 CD56 (NCAM) by Immunohistochemistry Brain Tumors, Sarcoma , Rhabdomyosarcomas, T/NK-Cell Lymphomas, Plasma Cell Dyscrasias, Tumor Markers, Lung Cancer Neural cell adhesion molecule (NCAM):
  • Expressed on natural killer cells and most neuroectodermal tissues
  • Retinoblastoma, medulloblastoma, astrocytoma, neuroblastoma, and rhabdomyosarcoma
2003592 CD57 by Immunohistochemistry T/NK-Cell Lymphomas, Tumor Markers Leu-7, HNK1:
  • Expressed by a subset of natural killer cells and some T-lymphocytes
  • Reacts with myelin-associated glycoprotein in neuroectodermal tissue
  • Aids in identifying small cell lung carcinoma, NK lymphocyte-mediated cytotoxicity, AIDS and AIDS-related complex (ARC), NK and T-cell subset tumors and neural tissue neoplasms
2003595 CD61 (Platelet Glycoprotein IIIA) by Immunohistochemistry Acute myelogenous leukemia, megakaryocytes Platelet glycoprotein IIIa:
  • Expressed on platelets and megakaryocytes
  • Specific for cells showing megakaryoblastic differentiation
  • Aids in identifying megakaryocytic/megakaryoblastic leukemias
2003598 CD68, KP1 by Immunohistochemistry Acute Myeloid Leukemia (AML), Monocytes, Histiocytes, Tumor Markers KP-1:
  • Expressed by Macrophages, blood monocytes, mast cells, and the cell population known as "plasmacytoid T-cells" in reactive lymph nodes
  • Strong to moderate staining in acute myeloid leukemia (AML), chronic myeloid leukemia (CML), true histiocytic neoplasia, melanoma, and some B-cell neoplasms (usually small lymphocytic lymphoma [SLL]) and weak staining in hairy cell leukemia
  • Weak staining in hairy cell leukemia
2003517 CD7 by Immunohistochemistry Sézary Syndrome, T/NK-Cell Lymphomas, Tumor Markers
  • Earliest T-cell-specific antigen to be expressed in lymphocytes
  • Present in thymocytes , most peripheral T-lymphocyte, most natural killer cells
  • Aids in identifying T-cell lymphomas and leukemias of T-cell origin
2003800 CD79A by Immunohistochemistry Acute Lymphoblastic Leukemia (ALL), Acute Myeloid Leukemia (AML), B-Cell Lymphomas, Tumor Markers
  • Expressed on pre B-cells until plasma cell stage
  • Found as intracellular component in plasma cells
  • Aids in identifying acute leukemia of precursor B-cell type (common ALL), B-cell lymphomas and some myelomas
2003520 CD8 by Immunohistochemistry T-Cell Lymphomas, Tumor Markers
  • Expressed on a cytotoxic/suppressor subset of T-lymphocyte natural killer cells, thymocytes, and on a subpopulation of null cells
  • Aids in identifying T-cell lymphomas/leukemias
2005534 CDK4 by Immunohistochemistry Sarcoma, liposarcoma
2003821 CDX2 by Immunohistochemistry Intestinal-type sinonasal adenocarcinoma, Colorectal Cancer, Tumor Markers, Lung Cancer
  • Exclusively marks nuclei of colonic epithelial cells and colorectal cancers
  • May be involved in the regulation of proliferation and differentiation in intestinal epithelial cells
  • May be used identifying metastatic colon carcinoma
2003830 Chromogranin A by Immunohistochemistry Neuroendocrine Tumors (NET), Carcinoid Tumors, Medullary Thyroid Cancer, Tumor Markers, Paraganglioma, Carotid body tumor, Olfactory neuroblastoma, pituitary adenomas, islet cell tumors, phaeochromocytomas, medullary thyroid carcinomas, Merkel cell tumors, and carcinoids
  • Expressed in neuronal cells and in secretory granules of endocrine cells: parathyroid gland, adrenal medulla, anterior pituitary gland, islet cells of the pancreas, and C-cells of the thyroid
  • Aids in identifying neuroendocrine tumors: pituitary adenomas, islet cell tumors, phaeochromocytomas, medullary thyroid carcinomas, Merkel cell tumors, and carcinoids
2003839 Collagen IV by Immunohistochemistry Alport Syndrome Renal disease, chronic kidney disease, hematuria, alport
  • Reacts with basement membranes in kidney, skin, striated and smooth muscle, spleen, lymph node, lung, placenta, and tendon
2013260 Comprehensive Muscle Biopsy Workup
2013261 Comprehensive Nerve Biopsy Workup
2008622 CXCL13 by Immunohistochemistry B-lymphocyte chemoattractant / B-Cell attracting chemokine-1 (BLC/BCA-1),
  • CXC chemokine family controlling the organization of B cells within follicles of lymphoid tissues such as spleen, lymph nodes and Peyer’s patches.
  • In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell.
  • Useful marker in the diagnosis of Angioimmunoblastic T-cell lymphoma, when used in a panel can differentiate it from other proliferative T-cell lymphoma.
2003842 Cyclin D1, SP4 by Immunohistochemistry Head and Neck Cancer, Tumor Markers, Mantle cell lymphoma, B-Cell Lymphomas
  • B-cell lymphoma-1
  • Mantle cell lymphoma, various carcinomas (strong staining in carcinomas), multiple myelomas, some parathyroid adenomas, and parathyroid carcinomas
2003845 Cytokeratin 19 (CK 19) by Immunohistochemistry Thyroid Cancer, Tumor Markers CK 19:
  • Reacts with a large number of epithelial cell types, including many ductal and glandular epithelia
  • Aides in identification of many benign and malignant epithelial lesions
2003848 Cytokeratin 20 (CK 20) by Immunohistochemistry Tumor Markers, Bladder Cancer, Hereditary Nonpolyposis Colorectal Cancer, HNPCC, Lung Cancer, Colorectal Cancer, Multiple Carcinomas , Merkel Cell, adenomas, gallbladder, bile duct, gastrointestinal adenocarcinoma, ductal cell adenocarcinomas of the pancreas, mucinous ovarian tumors, transitional cell carcinoma, Pancreatic carcinoma, cholangiocarcinoma, Mesothelioma Cytokeratin 20:
  • Expressed in intestinal epithelium, gastric foveolar epithelium, some endocrine cells of the upper portions of the pyloric glands, urethelium, and Merkel cells in epidermis
  • Aids in identifying colorectal carcinoma, adenomas of the gallbladder and bile ducts, ductal cell adenocarcinomas of the pancreas, mucinous ovarian tumors, transitional cell carcinomas, and Merkel cell carcinomas of the skin
  • Gastrointestinal adenocarcinomas express CK20 to a lesser degree
2003851 Cytokeratin 5,6 (CK 5,6) by Immunohistochemistry Mesothelioma, Bladder Cancer, Prostate Cancer (PSA), Lung Cancer, Tumor Markers Cytokeratins 5 and 6:
  • Stratified squamous epithelial cytokeratin
  • Aids in diagnosing low differentiated pavement epithelium carcinoma, adenocarcinoma, and mesothelioma
  • Differentiates epithelial mesotheliomas (positive-cytoplasmic staining with perinuclear enhancement) from lung adenocarcinoma (89% sensitive, 95% specific)
2003854 Cytokeratin 7 (CK 7) by Immunohistochemistry Gastrinoma, Mesothelioma, Bladder Cancer, Ovarian Cancer, Lung Cancer, Tumor Markers, Multiple Carcinomas, Breast Cancer Cytokeratin 7:
  • Reacts with most glandular and transitional epithelia: breast, lung, bladder, female genital tract (endometrium, fallopian tube), gastrointestinal tract (gallbladder, hepatic ducts, pancreatic ducts), urinary tract, and bile duct
  • Present with subtypes of ovarian, pulmonary, and breast adenocarcinomas, transitional cell carcinomas, tumors of female genital tract (endometrium, fallopian tube), urothelial carcinomas, breast carcinomas, and lung carcinomas
2003493 Cytokeratin 8,18 Low Molecular Weight (CAM 5.2) by Immunohistochemistry Carcinoid Tumors, Hepatocellular Carcinoma, Renal Cell, Rhabdoid tumors, Neuroendocrine, Pancreatic Cancer, Pheochromocytoma, Melanoma, Tumor Markers, Multiple Carcinomas, Colon Cancer, Gastrointestinal Cancer
  • Cytokeratin 8/18 , low molecular weight cytokeratin
  • Hepatocellular and renal cell carcinomas
  • Aids in identifying neuroendocrine carcinoma, melanomas (3%), nuclear inclusions of rhabdoid tumors (composed of tangled intermediate filaments made up of CK8 and vimentin), and mutations associated with idiopathic cirrhosis and chronic hepatitis
2003833 Cytomegalovirus (CMV) by Immunohistochemistry Cytomegalovirus Cytomegalovirus:
  • Reacts with the delayed and early DNA-binding protein p52
  • Does not crossreact with other herpesviruses or adenoviruses
2003857 D2-40 by Immunohistochemistry Mesothelioma, Brain Tumors
  • High sensitivity and specificity for lymphatic endothelium
  • Can be used as a reliable lymphatic endothelial cell marker in the evaluation of lymphatic involvement in tumors
2003863 Desmin by Immunohistochemistry Smooth Muscle, Reactive Mesothelial cells, leiomyosarcomas, myogenic sarcomas, rhabdomyosarcoma, PNET, neuroblastoma, GIST, gastrointestinal stromal tumor, Tumor Markers
  • Intermediate filament present in smooth and striated muscle
  • Expressed in reactive mesothelial cells, myoblasts, myofibroblasts (variable), endometrial stroma, and smooth muscle cells
  • Aids in idenitifying smooth muscle tumors (leiomyosarcomas), myogenic sarcomas, striated muscle tumors (rhabdomyosarcoma), PNET, neuroblastomas, and intra-abdominal desmoplastic small round cell tumors
2010168 DOG1 by Immunohistochemistry
  • Shown to be highly specific and sensitive in the diagnosis of GIST
  • Approximately 4–15% of GIST will stain weakly or be negative for CD117 by IHC; in the vast majority of these cases, DOG1 is expressed by IHC.
2003869 E-Cadherin by Immunohistochemistry Mesothelioma, Breast Cancer, Tumor Markers Cellular adhesion molecule:
  • Loss associated with invasive carcinoma
  • Differentiates LCIS from DCIS in indeterminate breast carcinoma
  • Reduced expression in invasive bladder cancer and ductal carcinoma
  • No expression in lobular carcinoma and LCIS
2003872 Epithelial Membrane Antigen (EMA) by Immunohistochemistry Adenocarcinoma, anaplastic large cell lymphomas, epitheloid sarcomas, Pagets’ disease, plasmacytomas, Pancreatic tumors, Tumor Markers Epithelial membrane antigen:
  • Prognostic
  • Expressed by almost all glandular and ductal epithelial cells including breast and pancreas, activated T-cells, monocytes, some B-cells, follicular dendritic cells, and perineurial cells
  • Aids in identifying most adenocarcinomas, anaplastic large cell lymphomas, epithelioid sarcomas, meningiomas, some mesotheliomas, myelomas, Paget disease, plasmacytomas, squamous cell tumors, and metastatic carcinomas
  • Associated with invasion in pancreatic tumors
2003875 Epithelial-related Antigen, MOC-31 by Immunohistochemistry Mesothelioma, Head and Neck Cancer, Lung Cancer adenocarcinomas, squamous cell carcinomas, adenomas, small cell lung cancers, carcinoids, adenocystic carcinomas, and carcinosarcomas Epithelial related antigen (MOC-31):
  • Aids in identifying adenocarcinomas, squamous cell carcinomas, adenomas, small cell lung cancers, carcinoids, adenocystic carcinomas, and carcinosarcomas
2007332 ERBB2 (HER2)(HercepTest) by Immunohistochemistry HERCEP IHC
  • Predicts a patient’s response to Herceptin Therapy
See Test Code 0049174 for HercepTest with Interpretation.
2012555 ERG by Immunohistochemistry
  • Prostate marker
  • May be used to aid in the identification of prostate adenocarcinomas through the detection of truncated ERG
2004516 Estrogen Receptor (ER) by Immunohistochemistry Ovarian Cancer, Breast Cancer, Tumor Markers Estrogen receptor-alpha:
  • Prognostic for breast cancer
  • Predictive for response of breast cancers to hormonal therapy
  • Differentiates endocervical from endometrial adenocarcinomas
2004055 Ewing Sarcoma (O13) by Immunohistochemistry Ewing Sarcoma, Sarcoma, PNET
  • MIC2 gene products
  • Glycoprotein HBA 71 antigen
  • Aids in identifying primitive peripheral neuroectodermal tumors, peripheral neuroepitheliomas, Ewing sarcoma and lymphoblastic lymphoma
2003878 Factor XIIIa by Immunohistochemistry Hepatocellular Carcinoma
  • megakaryocytes, and fibroblast-like mesenchymal or histiocytic cells present in the placenta, uterus, and prostate
  • Present in monocytes, macrophages, and dermal dendritic cells
  • Aids in differentiating dermatofibromas, dermatosarcoma protuberans, and desmoplastic malignant melanomas
  • Positive in capillary hemangioblastomas, hemangioendotheliomas, hepatocellular carcinomas, hemangiopericytomas, xanthogranulomas, glomus tumors, and meningiomas
2003896 Gastrin by Immunohistochemistry Gastrinoma, Pancreatic Neuroendocrine Tumors (NET), Carcinoid Tumors, Tumor Markers
  • Expressed in G-cells of the pyloric antrum
  • Aids in identifying G-cell hyperplasia and gastrin-secreting tumors
2012558 GATA3 by Immunohistochemistry
  • Breast marker
  • Can be used in a panel of antibodies for diagnosis of unknown primary carcinoma when carcinomas of the breast or bladder are a possibility. The pattern of reactivity should be nuclear.
2003899 Glial Fibrillary Acidic Protein (GFAP) by Immunohistochemistry Gastrointestinal Stromal Tumors (GIST), Brain Tumors Glial fibrillary acidic protein:
  • Expressed in astrocytes and some CNS ependymal cells
  • Identifies astrocytomas and ependymomas
  • Many neural tumors, such as neuroblastomas, schwannomas, and extra-CNS tumors, do not stain
2003905 Glucose Transporter-1 (GLUT-1) by Immunohistochemistry
  • Involved in glucose transport across epithelial and endothelial barrier tissues
  • Stains the membrane of normal erythrocytes in various normal and neoplastic tissues
2003908 Glycophorin A by Immunohistochemistry
  • Expressed in erythroid cells
  • Identifies M6 subtype of acute myeloblastic leukemia, erythroleukemia, and erythroblasts
2011925 Glypican 3 by Immunohistochemistry
  • Useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms’ tumor
2007173 Granzyme B by Immunohistochemistry
  • Granzyme B has been found to be expressed in the neoplastic counterparts of cytolytic CTL and NK-cells, therefore, Granzyme B may be a valuable tool in the diagnosis of T-cell/NK-cell lymphomas with cytotoxic phenotypes.
  • High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin’s disease.
2003860 Hairy Cell Leukemia, DBA.44 by Immunohistochemistry B-Cell Lymphomas
  • Developed against the B-cell antigen
  • Aids in identifying Hairy cell leukemia (particularly "hairy" cytoplasmic processes), some follicular center cell lymphomas, high grade B-cell lymphomas, splenic lymphomas with villous lymphocytes
2003914 HBME-1 (Mesothelial Cell) by Immunohistochemistry Mesothelioma, Thyroid Cancer Anti-mesothelial cell:
  • Has been demonstrated to immunostain the membrane and cytoplasm of normal plural and peritoneal mesothelial cells and of neoplastic epithelial mesothelioma cells.
  • Although both the membrane and cytoplasm of epithelial mesothelioma cells stain positive, the thick membrane staining pattern is found to be a more diagnostically useful marker of malignant mesothelioma.
2003941 Helicobacter pylori by Immunohistochemistry Gastric adenocarcinoma, gastric B-cell lymphoma persistent dyspepsia;duodenal ulcer, gastric MALT, early gastric cancer, gastritis
  • Reacts with antigens of the H. pylori organism
2003923 Hepatocyte Specific Antigen (HSA) by Immunohistochemistry Hepatocellular Carcinoma Hep Par-1:
  • Expressed in hepatocytes
  • Differentiates hepatocellular carcinomas and metastatic carcinomas
  • Differential diagnosis of hepatocellular carcinomas, cholangiocarcinomas, and hepatoblastomas
2003932 Herpes Virus 8 by Immunohistochemistry Kaposi Sarcoma, B-Cell Lymphomas, B- and T-Cell Markers Lymphoma, Castleman disease, Primary Effusion Lymphomas, Tumor Markers Human herpes virus type 8 (latent nuclearantigen):
  • Aids in identifying multicentric Castleman disease, angioimmunoblastic lymphadenopathies, and Kaposi sarcoma
2003935 Melanoma Antibody, HMB45 by Immunohistochemistry Melanoma, Tumor Markers, HMB45 Melanoma-specific antigen:
  • Expressed in junctional cells, blue nevus cells, and fetal and neonatal melanocytes
  • Reacts with the majority of melanomas and other tumors with melanoma/melanocytic differentiation, including melanotic schwannoma clear cell sarcoma
3000101 HSV Herpes Simplex Virus (HSV) Types I/II by Immunohistochemistry Herpes Simplex Virus (HSV)
  • Reacts with antigens common to HSV types 1 and 2; reacts with all the major glycoproteins present in the viral envelope
  • HSV I/II by IHC will aid in identifying tissue infected with the herpes simplex virus
2003920 Human Chorionic Gonadotropin (Beta-hCG) by Immunohistochemistry Trophoblastic, germ cell, lung, melanoma, gastrointestinal, ovarian, testicular cancer hCG:
  • Expressed on placental trophoblasts
  • Aids in identifying trophoblastic germ cell tumor
2003938 Human Placental Lactogen (HPL) by Immunohistochemistry Gestational Trophoblastic Disease, Products of Conception HPL:
  • Reacts with placental syncytrotrophoblastic cells
  • Identifies placental site trophoblastic tumors and exaggerated placental sites
2005857 IDH1R132H Mutation by Immunohistochemistry Glial tumors, astrocytomas , oligodendrogliomas, glioblastomas
  • Distinguishes primary from secondary Glioblastoma multiform (GBM)
  • IDH1 mutations occur in approximately 70% of astrocytomas and oligodendroglial tumors
  • Allows the highly sensitive and specific discrimination of various tumors, such as astrocytoma from primary glioblastomas or diffuse astrocytoma grade II from pilocytic astrocytoma or ependymoma
2003960 Immunoglobulin D (IgD) by Immunohistochemistry
  • Delta chains of IgD
2003963 Immunoglobulin G (IgG) by Immunohistochemistry
  • Gamma chains of IgG
2005844 Immunoglobulin G4 by Immunohistochemistry
  • IgG4-related sclerosing disease has been recognized as a systemic disease entity characterized by an elevated serum IgG4 level, sclerosing fibrosis and diffuse lymphoplasmacytic infiltration with the presence of many IgG4-positive plasma cells. It is important to recognize this entity and differentiate it from such mimics as lymphoma.
  • Immunohistochemical analysis in the cases of IgG4-related sclerosing disease exhibits significantly more IgG4 positive plasma cells in affected tissues. Clinical manifestations are apparent in the pancreas, bile duct, gallbladder, lacrimal gland, salivary gland, retroperitoneum, kidney, lung, breast thyroid, and prostate.
2003966 Immunoglobulin M (IgM) by Immunohistochemistry
  • Mu chains of IgM
2003969 Inhibin by Immunohistochemistry Brain Tumors, Ovarian Cancer
  • Expressed in Sertoli cells, granulosa cells, and prostate, brain, and adrenal cells
  • Present in sex-cord stromal tumors (including Sertoli cell tumors), adrenocortical tumors, placental and gestational trophoblastic lesions, granular cell tumors of gallbladder and extrahepatic bile ducts, and some carcinomas
2003448 INI1 (BAF47) by Immunohistochemistry Head and Neck Cancer, Sarcoma
  • Indicative of a tumor suppressor role
  • Heterozygous tumors in the soft tissues of the head and neck
2003975 MUM1/IRF4 by Immunohistochemistry B-Cell Lymphomas, Hodgkin lymphoma, Tumor Markers
  • Transcription factor required for B- and T-cell development
  • Expressed in a subset of diffuse large B-cell lymphomas and used in cell of origin classification
  • Aids in the differential diagnosis of PEL among other lymphomas involving the serous body cavities
2003981 Kappa Light Chains by Immunohistochemistry Plasma Cell Dyscrasias, B-Cell Lymphomas, plasma cell dyscrasias
  • Reacts with free kappa chains and those in intact immunoglobulin molecules
  • Restricted expression suggests monoclonality and neoplastic process
2003978 Keratin 903 (K903) High Molecular Weight by Immunohistochemistry Mesothelioma, Bladder Cancer, Prostate Cancer, Breast Cancer High molecular weight keratin, 34βE12:
  • Specific to prostate basal cells
  • Rules out prostatic adenocarcinoma
  • High grade PIN demonstrates K 903 staining
  • Identifies squamous cell carcinomas, urothelial carcinomas, adenocarcinomas of ductal origin (breast, pancreas), and small benign acinar lesions of the prostate
  • Aids in differentiating mesotheliomas from lung adenocarcinomas
2004519 Ki-67, MIB1, by Immunohistochemistry B-cell Lymphomas, T-Cell Lymphomas, Tumor marker, astrocytoma
  • Proliferation index indicator
  • Determines growth fraction
  • Aids in differentiating melanoma from nevus cells for sentinel node biopsy
  • Distinguishes benign and malignant adrenocortical tumors
2003984 Lambda Light Chains by Immunohistochemistry Plasma Cell Dyscrasias, B-Cell Lymphomas
  • Reacts with free lambda chains and those in intact immunoglobulin molecules
  • Restricted expression suggests monoclonality and neoplastic process
2013802 Langerin by Immunohistochemistry Indicated as an aid in the identification of Langerhans cells in the clinical differential diagnosis where Langerhans cell histiocytosis is suspected.
2003990 Lysozyme (Muramidase) by Immunohistochemistry Acute Myeloid Leukemia (AML)
  • Histiocytic and bacteriolytic enzyme
  • Present in granulocytes, monocytes, macrophages, histiocytes, normal hematopoietic cells, and some epithelial cells
  • Present in myeloid leukemias
2003993 Mast Cell Tryptase by Immunohistochemistry Mast Cell Disease
  • Present in secretory granules of mast cells
  • Present with inflammatory diseases
2003996 Melan A by Immunohistochemistry Melanoma, Tumor Markers
  • MART-1 antigen
  • Expressed in melanocytes, steroid-producing cells of the adrenal cortex, ovary, and testis
  • Identifies melanomas, adrenocortical tumors, Leydig tumors of the testis, and Sertoli-Leydig ovarian tumors
2011998 MITF by Immunohistochemistry
  • Used in the identification of melanotic lesions, such as malignant melanoma and melanotic neurofibroma
2004002 Muc-1 by Immunohistochemistry Glandular differentiation marker, Pancreatic, thyroid cancer, biliary, Salivary gland
  • Expressed in mucin-secreting epithelial cells of normal endometrium, kidney, lung, pancreas, and stomach
  • Utilized in the detection of Muc-1 glycoprotein in benign and malignant tumors
2004008 Muc-4 by Immunohistochemistry Colorectal Cancer, Tumor Markers, Pancreatic, thyroid, salivary gland, biliary
  • Stains stomach, colon, and the endothelial cells of small blood vessels and capillaries
  • Strong positive staining in colon polyps, colon carcinoma, and gastric adenocarcinoma
  • Positive staining also demonstrated in lung adenocarcinoma and ovarian mucinous adenocarcinoma
2004011 Muscle-Specific Actin (MSA) by Immunohistochemistry Sarcoma, leiomyomas, leiomyosarcomas, rhabdomyosarcomas, some pleomorphic liposarcomas, glomus tumors, desmoid tumors
  • Present in skeletal, cardiac, smooth muscle, and myoepithelial cells
  • Identifies soft tissue tumors with muscle differentiation (leiomyomas, leiomyosarcomas, and rhabdomyosarcomas), some pleomorphic liposarcomas, the majority of glomus tumors, occasional desmoid tumors, and myofibroblasts in some lesions
2004014 Myeloperoxidase (MPO) by Immunohistochemistry Myelodysplastic Syndrome (MDS), Acute Myelogenous Leukemia (AML), Tumor Markers MPO:
  • Reacts with myeloperoxidase from granulocytes
  • Aids in differentiating lymphoid leukemias from myeloid leukemias
  • Identifies granulocytic sarcomas
2004017 Myogenin (Myf4) by Immunohistochemistry Sarcoma, Tumor Markers, leiomyosarcomas, rhabdomyosarcomas
  • Expressed early in skeletal muscle differentiation.
  • Is a sensitive and specific marker for rhabdomyosarcoma.
  • Is more specific than desmin and muscle-specific actin and more sensitive than myoglobin.
2004031 Myoglobin by Immunohistochemistry Sarcoma, Tumor Markers, rhabdomyosarcomas
  • Oxygen binding protein
  • Expressed by striated muscle (cardiac, skeletal)
  • Present in rhabdomyosarcoma and other tumors with skeletal muscle differentiation
2004034 Myosin by Immunohistochemistry Sarcoma, Tumor Markers, rhabdomyosarcomas
  • Contractile protein
  • Expressed in smooth muscle (non-sarcomeric) and skeletal muscle (sarcomeric) forms
  • Aids in muscle differentiation
2008716 Napsin A by Immunohistochemistry
  • Napsin A is highly specific in adenocarcinomas of lung and is useful in distinguishing primary lung adenocarcinomas from adenocarcinomas of other organs
  • The pattern of reactivity is cytoplasmic
2004049 Neurofilament by Immunohistochemistry Tumor Markers
  • Cytoskeletal element in nerve axons/dendrites
  • Reacts with neurons, neuronal processes, peripheral nerves, sympathetic ganglion cells, and adrenal medulla
  • Identifies neuroblastoma and gangliomas
2004052 Neuron Specific Enolase, Polyclonal (NSE P) by Immunohistochemistry Neuroendocrine Marker, neuronal or neuroendocrine cells and their tumors: neuroblastomas and retinoblastomas Neuron-specific enolase:
  • Expressed by neuronal or neuroendocrine cells and their tumors: neuroblastomas and retinoblastomas
  • May label non-neuronal tumors: meningiomas, medulloblastomas, astrocytomas, glioblastomas, oligoastrocytomas, oligodendrogliomas, pituitary adenomas, schwannomas, ependymomas, meningosarcomas, gliosarcomas, small cell lung cancer, melanomas, and germ cell tumors
2004046 Neuronal Nuclei (NeuN) by Immunohistochemistry
  • Aids in the definitive identification of neuronal elements in ganglion cell tumors or hamartomas, in which a distinction between atypical glial cells and neurons may be difficult.
  • May be used, similarly, for the study of neuronal loss in epilepsy, neurodegenerative diseases, or other conditions
2004061 Octamer Transcription Factor-2 (Oct 2) by Immunohistochemistry B-Cell Lymphomas, Tumor Markers Octamer-binding transcription factor 2:
  • Aids in differentiating Hodgkin lymphomas (typically weak to negative) and B-cell lymphomas
2004058 Octamer Transcription Factor-3 and -4 (Oct 3/4) by Immunohistochemistry Testicular Cancer
  • Expressed by embryonic stem cells and germ cells.
  • Has been reported to be expressed in germ cell tumors and their metastases which exhibit features of pluripotentiality including seminoma/dysgerminoma/germinoma and embryonial carcinoma.
  • Has been proposed as a useful marker for germ cell tumors and to assist in establishing a germ cell origin for some metastatic tomors of uncertain primary origin.
2004064 p16 by Immunohistochemistry Head and Neck Cancer, Cervical Cancer, Bladder Cancer, Ovarian Cancer, Hereditary Nonpolyposis Colorectal Cancer, HNPCC, Colorectal Cancer, Tumor Markers, Pancreatic Cancer, Melanoma, prognostic F-12:
  • Negative regulator of the cell cycle
  • Prognostic significance (breast, colon, stomach, lung, and pituitary)
2004067 p21 (Waf1/Cip 1) by Immunohistochemistry Pancreatic Cancer, Melanoma, Bladder Cancer, Hereditary Nonpolyposis Colorectal Cancer, HNPCC, Colorectal Cancer, Tumor Markers WAF1/Cip1:
  • Inhibits and blocks cell cycle progression
  • Present in melanomas, pancreatic carcinomas, cervical carcinomas, thymomas, thyroid carcinomas, breast carcinomas, head and neck carcinomas, colon carcinomas, and Hodgkin lymphoma
2010142 P40 by Immunohistochemistry
  • More specific marker than p63 for lung SCC, bladder, breast, prostate, and head and neck cancers.
  • Selectively expressed in lung SCC.
2004076 P504S (AMACR) by Immunohistochemistry Prostate Cancer
  • Prostate
  • α-Methylacyl-CoA Racemase (AMCAR)
  • Specific for prostate adenocarcinomas
  • Detected in two premalignant lesions: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia
2004522 p53 by Immunohistochemistry Tumor marker, Non-small cell lung cancer, lung cancer
  • Tumor suppressor protein; prognostic indicator
2004073 p63 by Immunohistochemistry Bladder Cancer, Lung cancer, small cell lung cancer, neuroendrocrine
  • Differentiates prostatic adenocarcinoma and benign prostatic tissue.
  • Also distinguishes poorly differentiated squamous cell carcinoma from small cell carcinoma or adenocarcinoma.
2004094 Paired Helical Filament -Tau (PHF-Tau) by Immunohistochemistry
  • Tau is abnormally phosphorlated in Alzheimer´s disease
  • Main component in paired helical filaments (PHFs) and neurofibrillary tangles
2004118 Parathyroid Hormone (PTH) by Immunohistochemistry Acromegaly, Parathyroid Cancer, Tumor Markers, adenomas and primary and secondary hyperplasias PTH:
  • Reacts with parathyroid epithelial cells
  • Present in adenomas and primary and secondary hyperplasias
2004082 Pax-5 by Immunohistochemistry Acute Myelogenous Leukemia (AML), B-Cell Lymphomas, Hodgkin lymphomas, Tumor Markers
  • Member of the paired box family
  • B-cell-specific activator protein (BSAP)
  • Expressed in pro-, pre-, and mature B-cells, but not in plasma cells
  • Present in pre B-cell acute lymphoblastic leukemias and classic Hodgkin lymphomas
  • Aids in differential diagnosis of lymphoplasmacytic lymphomas or plasmacytomas
2010787 PAX8 by Immunohistochemistry
  • Expressed in a high percentage of ovarian serous, endometroid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas
  • Important marker of ovarian cancer and a useful marker for the differential diagnosis in lung and neck tumors, or tumors at distant sites where primary lung carcinoma, breast carcinoma, or thyroid carcinoma are possibilities.
2004085 PD-1 by Immunohistochemistry
  • Angioimmunoblastic T-cell lymphomas are the only hematopoietic tumors that are positive for PD1 protein.
  • In tonsil and lymph tissues the protein is expressed on T-cells and some B-cells of the light zone of germinal centers.
2011158 PD-L1 by Immunohistochemistry PD-L1 IHC
2010045 PIN4 Prostate Triple Stain by Immunohistochemistry Prostate Triple Stain:
  • Contains Basal Cell Cocktail (34βE12/p63) and P504s
  • Aids in the identification of prostate carcinoma
  • More sensitive than the individual staining of each antibody in the detection of prostatic basal cells
2004097 Placental Alkaline Phosphatase (PLAP) by Immunohistochemistry Testicular Cancer, Brain Tumors, Tumor Markers, Ovarian Cancer PLAP:
  • Expressed by placenta
  • Present in most germ cell tumors, and breast, lung, stomach, pancreas, and ovarian carcinomas
2004103 Pneumocystis jiroveci by Immunohistochemistry Pneumocystis jiroveci Pneumocystis jiroveci (pneumocystis carinii):
  • Detects presence of Pneumocystis jiroveci in infected tissue and free trophozoites
2004525 Progesterone Receptor (PR) by Immunohistochemistry Tumor Markers, Breast Cancer, Ovarian Cancer
  • Identifies A and B forms of progesterone
  • Predictive of response to hormone therapy for breast carcinoma and endometrial cancer
  • Aids in differentiating endocervical from endometrial adenocarcinomas
2004109 Prolactin by Immunohistochemistry Hypopituitarism, Tumor Markers
  • Produced in the anterior pituitary gland
  • Subclassifies pituitary adenomas
2004112 Prostate Specific Antigen by Immunohistochemistry Prostate Cancer, Tumor Markers Prostate-specific antigen:
  • Expressed by prostatic glandular epithelial cells and periurethral and perianal glands
  • Present in prostatic carcinomas, tumors of the colon, liver, lung, parotid, adrenal, and ovary, and, rarely, in metaplasias of the bladder walls
2004079 Prostatic Acid Phosphatase (PAP) by Immunohistochemistry Prostate Cancer Prostate acid phosphatase:
  • Reacts with prostatic epithelial cells and hyperplastic prostate
  • Present in carcinomas of the prostate and metastatic cells of prostate carcinoma, bladder carcinomas, and carcinoid tumors
2004091 Protein Gene Product (PGP) 9.5 by Immunohistochemistry Glucagonoma, Somatostatinoma, VIPoma, Ewing Sarcoma, Gastrinoma, Pancreatic Neuroendocrine Tumors (NET), Carcinoid Tumors, Pancreatic Cancer, Pheochromocytoma, Tumor Markers, neuronal neoplasis Protein gene product 9.5:
  • Expressed in neurons, neuroendocrine cells, and melanocytes
  • Present in neuronal neoplasias (carcinoid tumors)
2004124 Renal Cell Carcinoma (RCC) Antigen by Immunohistochemistry Renal cell carcinoma:
  • Localized along the brush border of the pars convolute and pars recta segments of the proximal tubule and focally along the luminal surface of Bowman?s capsule.
  • Of normal tissues, is also localized along the luminal surface of breast lobules and ducts, the luminal surface of the epididymal tubular epithelium, within the cytoplasm of the parathyroid parenchymal cells and focally within the colloid of thyroid follicles.
2004127 S-100 Protein by Immunohistochemistry Melanoma, Tumor Markers
  • Brain protein composed of S-100a and S-100b
  • Expressed in neural crest (Schwann cells, melanocytes, glial cells), chondrocytes, adipocytes, myoepithelial cells, macrophages, Langerhans cells, and dendritic cells
  • Present in 95% of melanomas (including desmoplastic and spindle cell tumors), 50% of malignant peripheral nerve sheath tumors, clear cell sarcomas, and occasional breast and undifferentiated carcinomas
2004137 Simian Virus 40 (SV-40) by Immunohistochemistry BK Virus, Simian Virus
  • Closely related to BK Virus and JC Virus
  • Used to identify all polymavirus infections due to cross-reactivity between SV-40 and BK Virus and JC Virus.
2006403 Smad4 by Immunohistochemistry
  • May be useful in the diagnosis of pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome
  • The pattern of reactivity is mostly cytoplasmic but sometimes nuclear expression is seen in many cell types with highest expression levels in placenta and gastrointestinal tract
2004130 Smooth Muscle Actin (SMA) by Immunohistochemistry Smooth Muscle Marker
  • Reacts with the alpha-smooth muscle isoform
  • Present in smooth muscle cells of vessels, parenchymes, myoepithelial cells, pericytes, and some stromal cells in the intestine, testis, and ovary
  • Aids in differentiating leiomyosarcoma from rhabdomyosarcoma
2012561 SOX11 by Immunohistochemistry
  • Lymphoma/hematopoietic marker
  • Will stain those cases of Mantle-cell lymphoma that are negative for the cyclin D1 stain, thereby aiding in a more timely diagnosis of MCL
2004139 Synaptophysin by Immunohistochemistry Neuroendocrine Marker, Glucagonoma, Somatostatinoma, VIPoma, Insulinoma, Ewing Sarcoma, Gastrinoma, Pancreatic Neuroendocrine Tumors (NET), Carcinoid Tumors, Brain Tumors, Pancreatic Cancer, Pheochromocytoma, Lung Cancer, Tumor Markers
  • Labels neuroendocrine cells and neurons in the brain, spinal cord, and retina
  • Present in neuroendocrine tumors and neuroendocrine tumors of epithelial type
2004148 T-cell Intracytoplasmic Antigen (TIA-1) by Immunohistochemistry T/NK-Cell Lymphomas, Tumor Markers T-cell intracytoplasmic antigen:
  • Reacts with 50-60% of CD8 lymphocytes, 10% of CD4 lymphocytes, monocytes, granulocytes, activated CD4 T-cells, activated NK cells, and con A-activated thymocytes
  • Aids in differentiating T-cell leukemias and lymphomas from B-cell leukemias and lymphomas
2004160 Tartrate-Resistant Acid Phosphatase (TRAP) by Immunohistochemistry Hairy cell leukemia, B-Cell Lymphomas
  • Found in hairy cells, osteoclasts, activated macrophages, and giant cells
  • Useful as a marker for hairy cell leukemia in bone marrow
2004142 TdT by Immunohistochemistry Acute Lymphoblastic Leukemia (ALL), B-cell and T-cell Acute Lymphoblastic Leukemia, Tumor Markers
  • Used in subtyping of blastic leukemias
  • Positive in all Acute Lymphoblastic Leukemia (ALL), except Burkitt's and B-cell FAB L-3
  • Positive in Lymphoblastic crisis of Chromic Myelogenous Leukemia (CML-BC-ALL) and Lymphoblastic Lymphoma
  • Some non-lymphocytic leukemias express positivity, but there is less intensity and greater variability
2010688 TFE3 by Immunohistochemistry
  • Indicated in the clinical diagnosis of malignancy as an aid in the recognition of Xp11 translocation in renal cell carcinoma and alveolar soft-part sarcoma.
  • Also reported in transitional renal cell carcinoma, lung adenocarcinoma, papillary thyroid carcinoma, melanoma, and mesothelioma.
2004145 Thyroglobulin by Immunohistochemistry Thyroid Cancer, Tumor Markers, Thyroid Disease
  • Protein synthesized by the follicular epithelial cells of the thyroid
  • Aids in the localization of thyroglobulin in hyperplastic and neoplastic thyroid and in monitoring of patients after treatment for follicular carcinomas
2004166 Thyroid Transcription Factor (TTF-1) by Immunohistochemistry Thyroid Cancer, Lung Cancer, Tumor Markers, sarcoma, mesothelioma, neuroendrocrine Thyroid transcription factor-1:
  • Expressed in lung and thyroid epithelial cells
  • Present in pulmonary small cell carcinomas, some pulmonary non-small cell carcinomas, papillary carcinomas, follicular carcinomas and goiter, thyroid medullary carcinomas, and thyroid papillary carcinomas
  • Aids in differentiating pulmonary adenocarcinomas from breast carcinomas
2004157 Toxoplasma gondii by Immunohistochemistry Toxoplasma gondii
  • Detects the presence of Toxoplasma gondii in infected tissues
2004169 Ubiquitin by Immunohistochemistry
  • Detects intracellular ubiquinated filamentous inclusions in the periphery of senile plaques and neurofibrillary tangles in Alzheimer disease and Lewy bodies in Parkinson disease
2004181 Vimentin by Immunohistochemistry Melanoma, Ovarian Cancer, Sarcoma, schwannoma, Endometrial Cancer, mesothelioma
  • Aids in identifying Melanomas and schwannomas
2004184 Wilms Tumor (WT1), N-terminus by Immunohistochemistry AML, Mesothelioma, Ovarian Cancer, desmoplastic round-cell tumor
  • Aids in identifying Wilms' tumor, mesotheliomas