Special Specimen Collection and Handling

QuantiFERON-TB Gold In-Tube (0051729)

Collect: At altitudes from sea level to 2,650 feet: QuantiFERON TB (Standard)Gold In-Tube collection kit.
At altitudes from 2,650-3,350 feet: QuantiFERON TB (Standard) Gold In-Tube collection kit.
At altitudes from 3,350-6,150 feet: QuantiFERON TB (High Altitude) Gold In-Tube collection kit.
At altitudes above 6,150 feet:
QuantiFERON TB (High Altitude) Gold In-Tube collection kit.
Specimen Preparation: At altitudes from sea level to 2,650 feet: Whole blood directly into each of the three tubes in the QuantiFERON TB (Standard) Gold In-Tube collection kit (ARUP
supply #45112) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
At altitudes from 2,650-3,350 feet: Collect 3 mL whole blood using a syringe. Transfer 1 mL (Min: 0.8 mL, Max 1.2 mL) to each of the three tubes in QuantiFERON TB
(Standard) Gold In-Tube collection kit (ARUP supply #45112).
At altitudes from 3,350-6,150 feet: Whole blood directly into each of the three tubes in the QuantiFERON TB (High Altitude) Gold In-Tube collection kit (ARUP 
supply #46261) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
At altitudes above 6,150 feet: Collect 3 mL whole blood using a syringe. Transfer 1 mL (Min: 0.8 mL, Max 1.2 mL) to each of the three tubes in QuantiFERON TB (High
Altitude) Gold In-Tube collection kit (ARUP supply #46261).
Tubes should be between 17°C and 25°C (63°F to 77°F) at the time of blood filling.
Immediately after filling tubes, shake them ten (10) times just firmly enough to ensure the entire inner surface of the tube is coated with blood, to solubilize antigens on
tube walls. Over-energetic shaking may cause gel disruption and could lead to aberrant results.
After mixing, incubate upright at 37° C for 16-24 hours (within 16 hours of collection).
After incubation, centrifuge for 15 minutes at 2000-3000 RCF (g).
After centrifugation, transport entire contents in each of the 3 original collection tubes.
Transport: Original collection tubes, after incubation and centrifugation, at 2–8°C.
Pediatric Collect/Transport: Original collection tubes, after incubation and centrifugation, at 2–8°C.
Remarks: Tubes should be between 17ºC and 25ºC (63ºF to 77ºF) at the time of blood filling. Immediately after filling tubes, shake them 10 times just firmly enough to ensure that the entire inner surface of the tube is coated with blood to solubilize antigens on tube walls. Overenergetic shaking may cause gel disruption and could lead to aberrant results.

After mixing, incubate upright at 37°C for 16–24 hours (within 16 hours of collection). If the blood is not incubated immediately after collection, remixing of the tubes by inverting 10 times must be performed immediately prior to incubation. After incubation, centrifuge for 15 minutes at 2000–3000 RCF (g).
Unacceptable Conditions: Room temperature specimens; specimens that have not been centrifuged.
Stability: After incubation and centrifugation—ambient: two hours; refrigerated: four weeks; frozen: unacceptable.

Collection Instructions for QuantiFERON-TB Gold In-Tube
Collection Instructions for QuantiFERON-TB Gold In-Tube (High Altitude; Elevations Between 3,350 and 6,150 Feet)

QuantiFERON-TB Gold In-Tube Instructional Video

Related Information

CORP-APPEND-0249J, Rev 5, October 2013