ARUP's Laboratory Test Directory

Influenza A Virus H1/H3 Subtyping by Real-Time RT-PCR : 2007469

Mnemonic: FLUTYPEPCR

Ordering Recommendation: Follow-up test for patients with documented influenza A. Not a first-line test for the detection of suspected influenza in most clinical situations. Detects and subtypes the 2 predominant strains of circulating influenza A (H1N1 and H3N2).
Methodology: Qualitative Reverse Transcription Polymerase Chain Reaction
Performed: Sun-Sat
Reported: 1-3 days
Specimen Required: Collect: Respiratory specimen: Bronchoalveolar lavage (BAL), nasal wash, nasopharyngeal swab, or pleural fluid.

Specimen Preparation: Fluid: Transfer 1 mL respiratory specimen to a sterile container. (Min: 0.5 mL) Also acceptable: Transfer to viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. Swabs: Place in viral transport media. Place each specimen in an individually sealed bag.

Storage/Transport Temperature: Refrigerated.

Remarks: Specimen source required.

Stability (collection to initiation of testing): Ambient: 8 hours; Refrigerated: 5 days; Frozen: 2 months

Interpretive Data: This test targets the H3 and 2009-H1 hemagglutinin genes. The current circulating Influenza A strains are detected and typed (H1N1 and H3N2), however, other H1 and H3 subtypes may also be detected.

This test is performed pursuant to an agreement with Roche Molecular Systems, Inc

See Compliance Statement B: www.aruplab.com/CS
CPT Code(s): 87502 Influenza A & B