ARUP's Laboratory Test Directory

Hepatitis C Virus High-Resolution Genotyping : 2006898

Mnemonic: HCV CORE

Ordering Recommendation: Do not order prior to molecular confirmation of positive hepatitis C virus (HCV) screen. Order before initiating HCV therapy to aid in determining therapy of choice, likelihood of response, and probable therapeutic duration. This assay should be used for prognosis and treatment selection when a higher level of subtype resolution is required (ie, non 6a/b vs. type 1 and type 1a vs. 1b).
Methodology: Polymerase Chain Reaction/Sequencing
Performed: Sun-Sat
Reported: 5-8 days
Specimen Required: Collect: Lavender (EDTA), pink (K2EDTA), plasma preparation tube, or serum separator tube (SST).

Specimen Preparation: Separate serum or plasma from cells within 6 hours. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature: Frozen.

Remarks: Please submit most recent viral load and test date if available.

Unacceptable Conditions: Heparinized specimens.

Stability (collection to initiation of testing): On cells: Ambient: 6 hours. After separation from cells: Refrigerated: 72 hours; Frozen: 4 months

Reference Interval:
By report
Interpretive Data: Hepatitis C viral RNA is assayed using reverse transcription polymerase chain reaction (RT-PCR) to amplify specific portions of both the Core and NS5B regions of the viral genome. The amplified nucleic acid is sequenced bi-directionally using dye-terminator chemistry (ABI). Sequencing data is compared to a database of characterized sequences.

Isolates of hepatitis C virus are grouped into six major genotypes (1-6). These genotypes are subtyped according to sequence characteristics. Sequencing both the Core and NS5B regions allows for subtyping of all confirmed and most provisional genotypes, including differentiation of 1a from 1b and typing of genotype 6.

This test is performed pursuant to a license agreement with Roche Molecular Systems, Inc.

See Compliance Statement B: www.aruplab.com/CS
Note: This test may be unsuccessful if the HCV RNA viral load is less than log 3.4 or 2500 IU/mL.
CPT Code(s): 87902
Cross References: HCV, Subtype (Hepatitis C Virus Genotyping) [Hepatitis C Virus High-Resolution Genotyping]