ARUP's Laboratory Test Directory

Human Immunodeficiency Virus (HIV) Combo Antigen/Antibody (HIV-1/O/2) by ELISA, with Reflex to HIV-1 Antibody Confirmation by Western Blot : 2006526

Mnemonic: HIV AGAB

Methodology: Qualitative Enzyme-Linked Immunosorbent Assay/Qualitative Western Blot
Performed: Wed, Sat
Reported: 1-5 days
Specimen Required: Collect: Serum separator tube. Also acceptable: Lavender (EDTA), pink (K2EDTA), green (sodium or lithium heparin), or red (clot activator)

Specimen Preparation: Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. Remove particulate material. (Min: 0.5 mL).

Storage/Transport Temperature: Refrigerated.

Remarks: Submit specimen according to Biological Substance, Category B, shipping guidelines.

Unacceptable Conditions: Specimens containing particulate material. Severely hemolyzed or heat-inactivated specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Indefinitely (avoid repeated freeze/thaw cycles)
Reference Interval:
 
Test Number Components Reference Interval
 Human Immunodeficiency Virus (HIV) Combo Antigen/Antibody (HIV-1/O/2) by ELISA Negative
0020284 Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot Negative

Interpretive Data: This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P). 
Note: This fourth-generation screen test is for the simultaneous qualitative detection of Human Immunodeficiency Virus Type 1 (HIV-1) p24 antigen and antibodies to HIV Type 1 (HIV-1 groups M and O) and HIV Type 2 (HIV-2), Results of the screen cannot be used to distinguish between the presence of HIV-1 p24 antigen, HIV-1 antibody, or HIV-2 antibody.

If the HIV-1,2 Combo Antigen/Antibodies screen is repeatedly reactive, then the Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot will be added. Additional charges apply.

Performed and Reported times indicated are for the screening portion of this test. Refer to Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot (ARUP test code 0020284) for additional information regarding Performed or Reported times for the reflex portion of this test.
CPT Code(s): 87389; if reflexed, add 86689
Cross References: 4th generation HIV screen; HIV Ag/Ab combination screen