| Ordering Recommendation: |
Molecular test for the detection of the BRAF V600E mutation in direct smear specimens from fine needle aspirate (FNA). For tissue block or formalin-fixed, paraffin-embedded cell blocks prepared from FNA, may use BRAF codon 600 Mutation Detection by Pyrosequencing (2002498).
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| Methodology: |
Polymerase Chain Reaction
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| Performed: |
Varies
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| Reported: |
7-10 days
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| Specimen Required: |
Collect: Tumor cells by Fine Needle Aspiration (FNA).
Specimen Preparation: Prepare FNA smear and Diff-Quick or equivalent stain by standard methods.
Storage/Transport Temperature: Two FNA slides (min: 1 slide) Transport slide(s) in a tissue transport kit (ARUP supply # 47808) available online through eSupply using ARUP Connect™or contact ARUP Client Services at (800) 522-2787.
Remarks: Include cytology report. For a general FNA collection and smear preparation refer to ARUP's Laboratory Test Directory: Cytology, Fine Needle Aspiration Collection at http://www.aruplab.com/guides/ug/tests/arup014.jsp
Unacceptable Conditions: Slides with less than 50 atypical or tumor cells.
Stability (collection to initiation of testing): Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
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