ARUP's Laboratory Test Directory

IDH1 and IDH2 Mutation Analysis, exon 4 : 2006444
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Additional Technical Information
  


Mnemonic: IDH1-2

Methodology: Polymerase Chain Reaction/Sequencing
Performed: Varies
Reported: 12-14 days
Specimen Required: Collect:  Lavender (EDTA) OR bone marrow (EDTA) OR tumor tissue.

Specimen Preparation:  Whole blood: Transport 5 mL (Min: 1 mL) OR Bone marrow: Transport 3 mL (Min: 1 mL) OR FFPE tumor tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or 4 unstained 5-micron thick slides (Min: 3 slides). Transport block and/or slid (s) in a tissue transport kit (ARUP supply # 47808) available online through eSupply using ARUP Connect™or contact ARUP Client Services at (800)522-2787.

Storage/Transport Temperature:  Whole blood OR Bone marrow: Refrigerated. FFPE tumor tissue: Room temperature or refrigerated. Ship in cooled containers during summer months.

Remarks:  For FFPE specimens include surgical pathology report. Tissue block will be returned after testing.

Unacceptable Conditions:  Whole blood OR Bone marrow: Serum or plasma. Specimens collected in anticoagulants other than EDTA. Frozen specimens. Clotted or grossly hemolyzed specimens. FFPE tumor tissue: No tumor in tissue. Specimens fixed/processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5). Decalcified specimens.

Stability (collection to initiation of testing):  Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
FFPE tumor tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable

Interpretive Data: Refer to report.

See Compliance Statement B: www.aruplab.com/CS
CPT Code(s): 81403 (2), G0452; For FFPE, add 88381
Cross References: Isocitrate dehydrogenase (IDH) 1 and 2 (IDH1 and IDH2 Mutation Analysis, exon 4)