ARUP's Laboratory Test Directory

B-Cell Clonality Screening (IgH and IgK) by PCR : 2006193
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Additional Technical Information
  


Mnemonic: BCELL SCRN

Methodology: Polymerase Chain Reaction/Capillary Electrophoresis
Performed: DNA isolation: Sun-Sat; Assay: Varies
Reported: 5-7 days
Specimen Required: Collect: Lavender (EDTA) OR bone marrow (EDTA) OR tissue.

Specimen Preparation: Whole blood: Transport 5 mL (Min: 1 mL) OR Bone marrow: Transport 3 mL (Min: 1 mL) OR Fresh tissue: Freeze immediately. Transport 100 mg or 0.5-2.0 cm3 tissue. OR FFPE tumor tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport frozen tissue in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature: Whole blood OR Bone marrow: Refrigerated. Fresh tissue: Frozen on dry ice. FFPE tumor tissue: Room temperature or refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions: Whole blood OR Bone marrow: Frozen specimens. Clotted or grossly hemolyzed specimens. FFPE tumor tissue: Specimens fixed/processed in alternative fixatives or heavy metal fixatives (B-4 or B-5) or tissue sections on slides. Decalcified specimens.

Stability (collection to initiation of testing): Whole blood OR Bone marrow: Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Fresh Tissue: Ambient: Unacceptable; Refrigerated: 2 hours; Frozen: 1 year
FFPE tumor tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Interpretive Data: Refer to report.

See Compliance Statement D: www.aruplab.com/CS
CPT Code(s): 81261, 81264, G0452