ARUP's Laboratory Test Directory

DNA Content/Cell Cycle Analysis, Hydatidiform Mole : 2006178

Mnemonic: DNA HYDAT

Methodology: Quantitative Flow Cytometry
Performed: Sun, Tue
Reported: 3-9 days
Specimen Required: Collect: Hydatidiform mole tissue.

Specimen Preparation: Paraffin embed tissue block with hydatidiform mole. If no normal tissue is included in the block, please supply a control tissue block to be processed in parallel.

Storage/Transport Temperature: Room temperature.

Remarks: Include H&E stained slide and surgical pathology report.

Unacceptable Conditions: No tissue remaining on the block. Specimens fixed in Bouin's Solution (picric acid), fixatives containing mercuric chloride (e.g. B5, Zenker's solution) or ethanol-based fixatives containing ethylene glycol, acetic acid, or zinc chloride. Decalcified specimens.

Stability (collection to initiation of testing): Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Interpretive Data: Flow Cytometry can be used to distinguish between partial and complete hydatidiform moles. Partial moles are usually triploid while complete moles are diploid or tetraploid. (Clinical Medicine: Pathology; 2008: 1; 61-7). Of the 35 cases of histologically apparent partial moles, no complication occurred in those that were triploid. However, 20 percent of those that were diploid had complications (persistence, metastasis). [Am J ObstetGynecol; 1987:157; 969-73].

See Compliance Statement B: www.aruplab.com/CS
Note: A thin section of each tissue submitted is stained with H&E. The DNA content is classified as diploid, triploid, tetraploid or aneuploid. The DNA index is the ratio of the DNA content of abnormal cells compared to normal cells.
CPT Code(s): 88182
Cross References: DNA Ploidy for Hydatidiform Moles (DNA Content/Cell Cycle Analysis, Hydatidiform Mole), Partial or Complete Hydatidiform Moles (DNA Content/Cell Cycle Analysis, Hydatidiform Mole)