| Ordering Recommendation: |
WT1 testing is appropriate for detecting mutations in exons 7 and 9 as well as for the presence of SNP rs16754 in cases of cytogenetically normal AML.
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| Methodology: |
Polymerase Chain Reaction/Sequencing
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| Performed: |
Varies
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| Reported: |
12-14 days
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| Specimen Required: |
Collect: Lavender (EDTA) OR bone marrow (EDTA).
Specimen Preparation: Transport 5 mL whole blood (Min: 1 mL) OR 3 mL bone marrow (Min: 1 mL).
Storage/Transport Temperature: Refrigerated
Unacceptable Conditions: Serum or plasma. Specimens collected in anticoagulants other than EDTA. Frozen specimens. Clotted or severely hemolyzed specimens
Stability (collection to initiation of testing): Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
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