ARUP's Laboratory Test Directory

High-Specificity Antiphospholipid Antibodies, IgG and IgM : 2005457

Mnemonic: HS APL

Ordering Recommendation: Acceptable initial test for strong suspicion of antiphospholipid syndrome (APS).
Methodology: Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Performed: Sun, Tue, Thu
Reported: 1-4 days
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Contaminated, heat-inactivated, grossly hemolyzed, grossly icteric, or severely lipemic specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen 1 year (avoid repeated freeze/thaw cycles)
Reference Interval:
 
Available Separately Components Reference Interval
No High-SpecificityAntiphospholipid Antibody, IgG 14 GPL or less: Negative
15-26 GPL: Indeterminate - Repeat testing in 12 weeks
27 GPL or greater: Positive
No High-SpecificityAntiphospholipid Antibody, IgM 14 MPL or less: Negative
15-37 MPL: Indeterminate - Repeat testing in 12 weeks
38 MPL or greater: Positive

Interpretive Data: High-specificity antiphospholipid IgG and IgM antibodies are directed against a mixture of phosphatidylserine, phosphatidic acid, and beta-2 glycoprotein 1 antigens. These antibodies are more specific than cardiolipin IgG and IgM antibodies in the diagnosis of antiphospholipid syndrome (APS).
Note: GPL = IgG phospholipid units; MPL = IgM phospholipid units
CPT Code(s): 83516 x 2