ARUP's Laboratory Test Directory

Paroxysmal Nocturnal Hemoglobinuria, WBC : 2005003
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Additional Technical Information
  


Mnemonic: PNH WBC

Ordering Recommendation: Use to quantify or monitor paroxysmal nocturnal hemoglobinuria clone size. Preferred initial diagnostic test is Paroxysmal Nocturnal Hemoglobinuria Panel, RBC and WBC (2005006).
Methodology: Quantitative Flow Cytometry
Performed: Sun-Sat
Reported: 1-4 days
Specimen Required: Patient Prep: New York State Clients: Testing is only approved for the Paroxysmal Nocturnal Hemoglobinuria Panel (ARUP test code 2005006) on whole blood specimens.

Collect: Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation: Transport 4 mL whole blood. (Min: 4 mL)

Storage/Transport Temperature: Refrigerated.

Remarks: Specimens must be analyzed within 72 hours of collection.
New York State Clients: Only the whole blood PNH PAN is acceptable and must be analyzed within 24 hours of collection if ambient or 48 hours of collection if refrigerated.

Unacceptable Conditions: Clotted, hemolyzed or frozen specimens. Bone marrow specimens. Specimens older than 72 hours.
New York State Clients: Specimens for the PNH PAN older than 24 hours ambient or older than 48 hours refrigerated. Clotted, hemolyzed or frozen specimens. Bone marrow specimens.

Stability (collection to initiation of testing): Ambient: 24 hours; Refrigerated: 72 hours; Frozen: Unacceptable
New York State Clients: PNH PAN-Ambient: 24 hours; Refrigerated: 48 hours. Frozen: Unacceptable
Reference Interval:
By report
Interpretive Data: This test was developed according to recently published guidelines (Cytometry B Clin Cytom. 2010 Jul; 78 (4):211-30),and includes diagnostic WBC analysis using FLAER, CD14, and CD24 as GPI-linked markers with CD15 and CD33 as lineage-specific markers. The lower limit of detection for this test is 0.1 percent PNH cells and is the most accurate measurement of the PNH clone size used to assist with therapeutic decisions in conventional PNH.

For initial diagnosis of PNH, Paroxysmal Nocturnal Hemoglobinuria Panel, RBC and WBC (ARUP test code 2005006) is available. This panel analyzes both RBCs and WBCs.

For delineation of RBC populations Types II and III when the PNH clone is 1 percent or greater, Paroxysmal Nocturnal Hemoglobinuria, High Sensitivity, RBC (ARUP test code 2004366) is available. This test also detects minor (0.005-0.999 percent) PNH clone populations found in bone marrow failure disorders.

See Compliance Statement A: www.aruplab.com/CS
CPT Code(s): 86356 x5
Cross References: PI-Linked Antigens, Monocytes and Granulocytes (Paroxysmal Nocturnal Hemoglobinuria, WBC)