ARUP's Laboratory Test Directory

Yeast Differentiation by PNA FISH : 2004769

Mnemonic: YST FISH

Methodology: Qualitative Peptide Nucleic Acid Fluorescence in situ Hybridization
Performed: Sun-Sat
Reported: Within 24 hours
Specimen Required: Collect: Client laboratory identified positive blood culture bottle with yeast from an automated or manual system.

Specimen Preparation: Transfer 5 mL aliquot of blood from yeast-positive blood culture bottle to a sterile transport tube. Also acceptable: A yeast-positive blood culture bottle. (Min: 1 mL)

Storage/Transport Temperature: Room temperature.

Remarks: Provide any stain results performed at client laboratory.

Unacceptable Conditions: Negative blood culture. Whole blood, body fluid, or slides.

Stability (collection to initiation of testing): Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Interpretive Data: Candida krusei is intrinsically resistant to fluconazole. C. glabrata and C. tropicalis may be resistant to fluconazole. Consult your institution's antibiogram or an infectious disease physician for guidance on empiric therapy.
Note: This is a presumptive result; definitive yeast identification should be performed from subculture.

For fungal identification, refer to Fungal (Mould/Yeast) Identification (ARUP test code 0060163).  For fungal susceptibility, refer to Antimicrobial Susceptibility - Yeast Susceptibility (ARUP test code 0060235).
CPT Code(s): 87149