ARUP's Laboratory Test Directory

Bordetella pertussis DFA : 2004667

Mnemonic: PERTFA

Ordering Recommendation: Not recommended for the diagnosis of pertussis due to low sensitivity. CDC first-line tests for pertussis are PCR (0065080) and/or culture (0060117).
Methodology: Direct Fluorescent Antibody Stain
Performed: Sun-Sat
Reported: Within 24 hours
Specimen Required: Collect: Nasal aspirate or washing or nasopharyngeal swab.

Specimen Preparation: Aspirate or Washing: Transfer to a sterile container.(Min: 0.5 mL)
Nasopharyngeal Swab:
Place swab in Regan-Lowe transport media and incubate for 24-48 hours at 35°C (ARUP supply# 24962). Available online
through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. Also acceptable: Swab in Jones Kendrick media or Aimes
with charcoal media, or swabs in described media sent directly without incubation.
If both culture and DFA are ordered, two swabs or swab plus slides are required.


Storage/Transport Temperature: Room temperature or refrigerated.

Remarks: Source of specimen is preferred.

Unacceptable Conditions: Sputum. Swabs or media other than listed above.

Stability (collection to initiation of testing): Incubated Regan-Lowe: Ambient: 4 days; Refrigerated: 4 days; Frozen: Unacceptable.
Unincubated Regan-Lowe, Aimes with charcoal swab, Aspirate or Washing:
Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable.
Slides:
Ambient: 1 week; Refrigerated: 1 week; Frozen: 1 month


Reference Interval:
Negative 
Interpretive Data: DFA is not recommended for diagnosing infections caused by B. pertussis. Refer instead to Bordetella pertussis Culture (ARUP test code 0060117), or Bordetella pertussis/parapertussis by PCR (ARUP test code 0065080) for amplified DNA testing of respiratory specimens.
CPT Code(s): 87265
Cross References: Pertussis Smear (Bordetella pertussis DFA) , Whooping Cough (Bordetella pertussis DFA)