ARUP's Laboratory Test Directory

HIV-1 Integrase Inhibitor Resistance by Sequencing : 2004457

Mnemonic: HIV1INT

Methodology: Reverse Transcription Polymerase Chain Reaction/Sequencing
Performed: Tue, Fri
Reported: 7-10 days
Specimen Required: Collect: Lavender (EDTA), pink (K2EDTA), or plasma preparation tube.

Specimen Preparation: Separate plasma from cells within 6 hours. Transfer 4 mL plasma to an ARUP Standard Transport Tube. (Min: 2.5 mL)

Storage/Transport Temperature: Frozen.

Remarks: Viral load required.

Unacceptable Conditions: Serum. Heparinized specimens.

Stability (collection to initiation of testing): On cells: Ambient: 6 hours; After separation from cells: Ambient: 24 hours; Refrigerated: 5 days; Frozen: 4 months

Reference Interval:
By report
Interpretive Data: The entire integrase-encoding region is sequenced. Mutations associated with resistance to integrase inhibitors are reported. Mutations in viral sub-populations below 20 percent of total may not be detected.

Refer to Statement D under Testing Information at http://www.aruplab.com.
Note: This test may be unsuccessful if the plasma HIV-1 RNA viral load is less than 500 HIV-1 RNA copies per mL of plasma.
CPT Code(s): 87906 
Cross References: HIV Integrase Genotype (HIV-1 Integrase Inhibitor Resistance by Sequencing), Raltegravir (Isentress™) Resistance (HIV-1 Integrase Inhibitor Resistance by Sequencing)
 
 

 

 

 
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