#ExistInterpData>This assay tests for CD59 on erythrocytes using flow cytometry. The percentage of red blood cells (RBCs) with normal expression of CD59 is decreased in paroxysmal nocturnal hemoglobinuria (PNH). The presence of a subclinical (0.005 percent-0.999 percent) PNH population in myelodysplastic bone marrow disorders such as aplastic anemia or refractory anemia may correlate with a positive immunotherapeutic response. (Blood 2006; 107, 1308-1314).
The lower limit of detection of this test is 0.005 percent PNH cells and distinguishes between Type II and Type III cells when the PNH cell percentage is 1 percent or greater. Glycophorin A is used to gate the RBC population. CD59 is the GPI-linked antigen. Recent RBC transfusions may decrease the percentage of PNH cells. (Cytometry 42:223-33, 2000).
For the most accurate measurement of the PNH clone size, the PNH WBC (ARUP test code 2005003) is available to assist with therapeutic decisions in conventional PNH.
For initial diagnosis of PNH and analysis of both RBCs and WBCs, Paroxysmal Nocturnal Hemoglobinuria Panel, RBC and WBC (ARUP test code 2005006) is available.
See Compliance Statement A: www.aruplab.com/CS
||PI-Linked Antigens on RBCs (Paroxysmal Nocturnal Hemoglobinuria, High Sensitivity, RBC)