ARUP's Laboratory Test Directory

Bordetella pertussis Antibodies, IgA, IgG, and IgM by Immunoblot : 2004328

Mnemonic: BORD BLOTS

Ordering Recommendation: If serology is used to assess late-stage pertussis, the recommended test is Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot (2001774). CDC first-line tests for pertussis are PCR (0065080) and/or culture (0060117).
Methodology: Qualitative Immunoblot
Performed: Tue
Reported: 1-8 days
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Heat-inactivated specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year
Reference Interval:
 
Bordetella pertussis Antibodies, IgA, IgG, and IgM by Immunoblot
Effective February 19, 2013
 Components Reference Interval
Bordetella pertussis Antibody, IgG by Immunoblot   
 Bordetella pertussis Ab, IgG by Immunoblot Interp Negative
 B. pertussis, IgG Immunoblot PT100 Negative
 B. pertussis, IgG Immunoblot PT Negative
 B. pertussis, IgG Immunoblot FHA Negative
Bordetella pertussis Antibody, IgA by Immunoblot   
 Bordetella pertussis Ab, IgA by Immunoblot Interp Negative
 B. pertussis, IgA Immunoblot PT Negative
 B. pertussis, IgA Immunoblot FHA Negative
Bordetella pertussis Antibody, IgM by Immunoblot   
 Bordetella pertussis Ab, IgM by Immunoblot Interp Negative
 B. pertussis, IgM Immunoblot PT Negative
 B. pertussis, IgM Immunoblot FHA Negative

Note: This assay tests for the presence of pertussis toxin (PT), pertussis toxin PT 100 (PT-100), and filamentous hemagglutinin antibody (FHA).
CPT Code(s): 86615 x3
Cross References: Pertussis Antibody (Bordetella pertussis Antibodies, IgA, IgG & IgM by Immunoblot) , Whooping Cough (Bordetella pertussis Antibodies, IgA, IgG & IgM by Immunoblot)