ARUP's Laboratory Test Directory

Bordetella pertussis Antibody, IgG by Immunoblot : 2004327

Mnemonic: BORDG BLOT

Ordering Recommendation: If serology is used to assess late-stage pertussis, the recommended test is Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot (2001774). CDC first-line tests for pertussis are PCR (0065080) and/or culture (0060117).
Methodology: Qualitative Immunoblot
Performed: Tue
Reported: 1-8 days
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Heat-inactivated specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year
Reference Interval:
 
Bordetella pertussis Antibody, IgG by Immunoblot
Effective February 19, 2013
Components Reference Interval
Bordetella pertussis Ab, IgG by Immunoblot Interp Negative
B. pertussis, IgG Immunoblot PT100 Negative
B. pertussis, IgG Immunoblot PT Negative
B. pertussis, IgG Immunoblot FHA Negative

Note: This assay tests for the presence of pertussis toxin (PT), pertussis toxin PT 100 (PT-100), and filamentous hemagglutinin antibody (FHA).
CPT Code(s): 86615
Cross References: Pertussis Antibody (Bordetella pertussis Antibody, IgG by Immunoblot) , Whooping Cough (Bordetella pertussis Antibody, IgG by Immunoblot)