ARUP's Laboratory Test Directory

Bordetella pertussis Antibody, IgA by Immunoblot : 2004316

Mnemonic: BORDA BLOT

Ordering Recommendation: Not a stand-alone test. CDC first-line tests for pertussis are PCR (0065080) and/or culture (0060117). If serology is used to assess late-stage pertussis, the recommended test is Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot (2001774).
Methodology: Qualitative Immunoblot
Performed: Tue
Reported: 1-8 days
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Contaminated or heat-inactivated specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year

Reference Interval:
 
Bordetella pertussis Antibody, IgA by Immunoblot
Effective February 19, 2013
Components Reference Interval
Bordetella pertussis Ab, IgA by Immunoblot Interp Negative
B. pertussis, IgA Immunoblot PT Negative
B. pertussis, IgA Immunoblot FHA Negative

Note: This assay tests for the presence of pertussis toxin (PT) and filamentous hemagglutinin antibody (FHA).
CPT Code(s): 86615
Cross References: Pertussis Antibody (Bordetella pertussis Antibody, IgA by Immunoblot) , Whooping Cough (Bordetella pertussis Antibody, IgA by Immunoblot)