Influenza A H1N1 (2009) Oseltamivir Resistance by Sequencing : ARUP Lab Tests

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Influenza A H1N1 (2009) Oseltamivir Resistance by Sequencing : 2004218

Mnemonic: H1N1 RES

Methodology:	Reverse Transcription Polymerase Chain Reaction/Pyrosequencing
Performed:	Sun-Sat
Reported:	1-2 days
Specimen Required:	Collect: Respiratory specimen: Broncheoalveolar lavage (BAL), bronchial aspirate, bronchial wash, endotracheal aspirate, endotracheal wash, nasal swab, nasopharyngeal swab, nasal aspirate, sputum, throat swab or tracheal aspirate. Specimen Preparation: Transfer 1 mL respiratory specimen to viral transport media (ARUP supply #12884). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. (Min: 0.5 mL). Place each specimen in an individually sealed bag. Storage/Transport Temperature: Refrigerated. Remarks: Specimen source required. Unacceptable Conditions: Dry swabs. Stability (collection to initiation of testing): Ambient: 8 hours; Refrigerated: 10 days; Frozen: 2 months

Interpretive Data:	This test is performed pursuant to a license agreement with Roche Molecular Systems, Inc. Refer to Statement B under Testing Information at http://www.aruplab.com.
Note:	This test detects H275Y, a single-point mutation in the neuraminidase gene of 2009 H1N1 influenza A, which is known to confer resistance to oseltamivir. This test will not detect the H275Y mutation in other influenza A strains. Prior to ordering this test, the patient should have tested positive for 2009 H1N1. This test is not intended for disease detection or for typing of influenza A strains.
CPT Code(s):	83891 Isolation; 83898 Amplification; 83894 Separation; 83904 Sequencing; 83912 Interpretation and report
Cross References:	Influenza Virus H1N1 (Influenza A H1N1 (2009) Oseltamivir Resistance by Sequencing)

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