ARUP's Laboratory Test Directory

NRAS Mutation Detection by Pyrosequencing : 2003123
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Additional Technical Information
  


Mnemonic: NRAS

Methodology: Polymerase Chain Reaction/Pyrosequencing
Performed: Sun-Sat
Reported: 10-12 days
Specimen Required: Collect: Tumor tissue.

Specimen Preparation: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or 5 unstained 5 micron slides. (Min: 3 slides). Transport block and/or slide(s) in a tissue transport kit (ARUP supply # 47808) available online through eSupply using ARUP Connect™or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature: Room temperature. Ship in cooled container during summer months.

Remarks: Include surgical pathology report. Tissue block will be returned after testing.

Unacceptable Conditions: Frozen specimens. Specimens fixed/processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5). Decalcified specimens.

Stability (collection to initiation of testing): Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Interpretive Data: Refer to report.

See Compliance Statement B: www.aruplab.com/CS
Note: This assay detects mutations in codons 12, 13, and 61.
CPT Code(s): 88381, 81404, G0452
Cross References: N-RAS (NRAS Mutation Detection by Pyrosequencing) , RAS Mutation Analysis, Thyroid Cancer (NRAS Mutation Detection by Pyrosequencing)