ARUP's Laboratory Test Directory

HLA-DR Genotyping : 2002798
[ image for: HLA Test Request Form Recommended]
HLA Test Request Form Recommended
  


Mnemonic: HLA-DR DNA

Ordering Recommendation: Do not use for specific disease screening or diagnosis (eg , celiac disease, rheumatologic diseases).
Methodology: Polymerase Chain Reaction/Sequence Specific Oligonucleotide Probe Hybridization
Performed: Mon-Fri
Reported: 3-7 days
Specimen Required: Collect: Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B).

Specimen Preparation: Transport 5 mL whole blood. (Min: 3 mL)

Storage/Transport Temperature: Room temperature.

Unacceptable Conditions: Specimens collected in green (sodium or lithium heparin).

Stability (collection to initiation of testing): Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable
Reference Interval:
By report
Interpretive Data: Background Information for HLA-DR Genotyping:
Purpose: For immunization/vaccination trials or to aid the clinical diagnosis of diseases strongly associated with the HLA-DR locus.
Methodology: PCR followed by Sequence Specific Oligonucleotide Probe Hybridization of HLA-DR locus.
Analytical Sensitivity & Specificity: Medium to high resolution of HLA-DR locus.
Limitations: The presence of a disease-associated HLA combination does not establish a diagnosis. If less than 2 alleles are reported for a locus, the patient is likely homozygous. Rare diagnostic errors can occur due to primer or probe site mutations. This test is not sufficient for comprehensive HLA evaluation for clinical hematopoietic stem cell transplantation (refer to HLA Bone Marrow Transplantation Evaluation - 2002806).

Counseling and informed consent are recommended for genetic testing. Consent forms are available online at www.aruplab.com.
Note: Order this test for single antigen HLA-DR identification.  Please specify antigens.  Refer to HLA-DQB Genotyping for single antigen HLA-DQB identification.
CPT Code(s): 81382