ARUP's Laboratory Test Directory

Tramadol and Metabolites - Confirmation/Quantitation - Serum or Plasma : 2002764

Mnemonic: TRAMAD SP

Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Performed: Tue
Reported: 1-7 days
Specimen Required: Collect: Gray (sodium fluoride/potassium oxalate). Also acceptable: Plain red, green (sodium heparin), lavender (EDTA), or pink (K2EDTA).

Specimen Preparation: Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Separator tubes. Plasma or whole blood collected in lt. blue (sodium citrate). Specimens exposed to repeated freeze/thaw cycles.

Stability (collection to initiation of testing): After separation from cells: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 3 years
Interpretive Data: Interpretive Data: Methodology: LC-MS/MS

Drugs covered: Tramadol, n-desmethyltramadol (qualitative only), and o-desmethyltramadol (qualitative only).

Positive cutoff:
Tramadol: 50 ng/mL
n-desmethyltramadol: 100 ng/mL
o-desmethyltramadol: 100 ng/mL

For medical purposes only; not valid for forensic use.

The presence of metabolite(s) without parent drug may indicate use of parent drug during the prior week.

The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

See Compliance Statement B: www.aruplab.com/CS
CPT Code(s): 83925
Cross References: Pain Management (Tramadol & Metabolites - Confirmation/Quantitation - Serum or Plasma), Topalgic (Tramadol & Metabolites - Confirmation/Quantitation - Serum or Plasma), Tramadol (Tramadol & Metabolites - Confirmation/Quantitation - Serum or Plasma) , Tramal (Tramadol & Metabolites - Confirmation/Quantitation - Serum or Plasma), Ultracet (Tramadol Component) (Tramadol & Metabolites - Confirmation/Quantitation - Serum or Plasma), Ultram (Tramadol & Metabolites - Confirmation/Quantitation - Serum or Plasma), Zamudol (Tramadol & Metabolites - Confirmation/Quantitation - Serum or Plasma)