ARUP's Laboratory Test Directory

Buprenorphine and Metabolites - Confirmation/Quantitation - Serum/Plasma : 2002742

Mnemonic: BUPREN SP

Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Performed: Sun-Sat
Reported: 1-4 days
Specimen Required: Collect: Gray (sodium fluoride/potassium oxalate). Also acceptable: Plain red, green (sodium heparin), lavender (EDTA), or pink (K2EDTA).

Specimen Preparation: Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Separator tubes. Plasma or whole blood collected in lt. blue (sodium citrate). Specimens exposed to repeated freeze/thaw cycles.

Stability (collection to initiation of testing): After separation from cells: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 3 years
Interpretive Data: Methodology: LC-MS/MS

Drugs covered: Buprenorphine, norbuprenorphine, buprenorphine glucuronide (qualitative only), norbuprenorphine glucuronide (qualitative only).

Positive cutoff:
Buprenorphine: 2 ng/mL
Norbuprenorphine: 2 ng/mL
Buprenorphine glucuronide: 5 ng/mL
Norbuprenorphine glucuronide: 5 ng/mL

For medical purposes only; not valid for forensic use.

The presence of metabolite(s) without parent drug may indicate use of parent drug during the prior week.

The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

See Compliance Statement B: www.aruplab.com/CS
CPT Code(s): 83925 
Cross References: Buprenex (Buprenorphine & Metabolites - Confirmation/Quantitation - Serum/Plasma), Buprenorphine (Buprenorphine & Metabolites - Confirmation/Quantitation - Serum/Plasma), Buprenorphine and Metabolite, Serum (Buprenorphine & Metabolites - Confirmation/Quantitation - Serum/Plasma) , Buprenorphine Glucuronide (Buprenorphine & Metabolites - Confirmation/Quantitation - Serum/Plasma), Butrans (Buprenorphine & Metabolites - Confirmation/Quantitation - Serum/Plasma), Norbuprenorphine (Buprenorphine & Metabolites - Confirmation/Quantitation - Serum/Plasma), Norbuprenorphine Glucuronide (Buprenorphine & Metabolites - Confirmation/Quantitation - Serum/Plasma) , Norspan (Buprenorphine & Metabolites - Confirmation/Quantitation - Serum/Plasma), Pain Management (Buprenorphine & Metabolites - Confirmation/Quantitation - Serum/Plasma), Suboxone (Buprenorphine & Metabolites - Confirmation/Quantitation - Serum/Plasma), Subutex (Buprenorphine & Metabolites - Confirmation/Quantitation - Serum/Plasma), Temgesic (Buprenorphine & Metabolites - Confirmation/Quantitation - Serum/Plasma), Transtec (Buprenorphine & Metabolites - Confirmation/Quantitation - Serum/Plasma), Vetergesic (Buprenorphine & Metabolites - Confirmation/Quantitation - Serum/Plasma)